RT Journal Article
T1 Colloidal centrifugation of stallion semen results in a reduced rate of sperm DNA fragmentation
A1 Gosálvez Berenguer, Jaime
A1 Crespo Castejón, Francisco
A1 Gutiérrez Cepeda, Luna
A1 Serres Dalmau, María Consolacion
A1 Jonhnston, Stephen
AB Stallion spermatozoa recovered and examined immediatelyafter colloidal centrifugation resulted in a higher straight-linevelocity (VSL) than sperm processed using direct conven-tional  centrifugation  (p = 0.000),  but  there  was  nodifferences  in  the  progressive  motility  or  sperm  DNAfragmentation (SDF) as determined by the sperm chromatindispersion assay. However, when centrifuged spermatozoawere incubated at 37°C for 24 h to determine the rate of SDF(r-SDF), a lower r-SDF (p = 0.0011) was observed in thosesperm recovered after colloidal separation (0.5 ± 0.1%⁄h)compared to direct (1.2 ± 0.4%⁄h) or no centrifugation (r-SDF = 1.2 ± 0.3%⁄h). These results confirm that colloidalseparation of stallion spermatozoa results in prolonged spermDNA longevity, but these differences were only apparentfollowing a period of incubation and dynamic assessment.Consequently,  we  strongly  recommend  the  use  of  thedynamic form of the SDF assay for evaluating centrifugationand⁄or otherex vivoprocedures, as a single basal assessmentof SDF may inadvertently result in a false-positive evaluationof DNA quality.
PB Wiley
SN 0936-6768
YR 2013
FD 2013-04
LK https://hdl.handle.net/20.500.14352/97263
UL https://hdl.handle.net/20.500.14352/97263
LA eng
NO 8)	Crespo F, Gosalvez J, Gutiérrez-Cepeda L, Serres C, Johnston SD. Colloidal centrifugation of stallion semen results in a reduced rate of sperm DNA fragmentation. Reprod Domest Anim. 2013 Apr;48(2):e23-5.
DS Docta Complutense
RD 18 jul 2024