RT Journal Article
T1 Combinations of PBPs and MurM protein variants in early and contemporary high-level penicillin-resistant Streptococcus pneumoniae isolates in Spain
A1 Del Campo, Rosa
A1 Cafini, Fabio
A1 Morosini, María Isabel
A1 Fenoll, Asunción
A1 Liñares, Josefina
A1 Alou Cervera, Luis
A1 Sevillano Fernández, David
A1 Cantón Moreno, Rafael María
A1 Prieto Prieto, José
A1 Baquero, Fernando
AB Objectives: High-level penicillin resistance in Streptococcus pneumoniae requires extensive re-modulation of the penicillin-binding proteins (PBPs), and murM gene function is also required for the expression of resistance. In this work, we determined whether specific changes in PBPs were associated with specific MurM variants.Methods: Two collections of highly penicillin-resistant (MIC 2-8 mg/L) isolates, including 10 early (1997-1998) and 23 contemporary (2002-2004) isolates, were studied.Results: Most of the isolates belonged to clones Spain(6B)-2 (13 strains), Spain(23F)-1 (10 isolates) and Spain(14)-5 (20 isolates). Different protein variants of MurM (MA, MB5, MB6, MB9 and MB10), PBP1A (A-C), PBP2B (A-D) and PBP2X (A-C) were recognized, including two murM alleles not previously described. Particular [MurM-PBP1A-2B-2X] allelic combinations were predominant among the different clones, including [MA-B-B-B] for old (MIC 2 mg/L) and [MB10-C-A-B] for recent (MIC 4-8 mg/L) Spain(6B)-2 isolates, [MA-A-C-A] for Spain(23F)-1 and [MB5-A-A-A] in Spain(14)-5 isolates.Conclusions: Although S. pneumoniae has a basic recombinational population structure, our results indicate remarkable conservation of PBPs and MurM protein types within each clone. This suggests that particular PBPs-MurM combinations tend to be preserved and may have an independent evolutionary history in particular clones.
PB Oxford University Press
SN 0305-7453
YR 2006
FD 2006-03-13
LK https://hdl.handle.net/20.500.14352/107170
UL https://hdl.handle.net/20.500.14352/107170
LA eng
NO del Campo R, Cafini F, Morosini MI, Fenoll A, Liñares J, Alou L, Sevillano D, Cantón R, Prieto J, Baquero F; Spanish Pneumococcal Network (G3/103). Combinations of PBPs and MurM protein variants in early and contemporary high-level penicillin-resistant Streptococcus pneumoniae isolates in Spain. J Antimicrob Chemother. 2006 May;57(5):983-6.
DS Docta Complutense
RD 5 abr 2025