RT Journal Article
T1 Capsular type diversity of Mannheimia haemolytica determined by multiplex real-time PCR and indirect hemagglutination in clinical isolates from cattle, sheep, and goats in Spain
A1 Arnal, José Luis
A1 Fernández, Ana
A1 Vela Alonso, Ana Isabel
A1 Sanz, Celia
A1 Fernández-Garayzábal Fernández, José Francisco
A1 Cid Vázquez, María Dolores
AB This study compares the utility of a commercially available multiplex q-PCR assay for serotyping A1, A2, and A6 M. haemolytica serotypes with indirect hemagglutination, for determining the relative distribution of M. haemolytica capsular types associated with respiratory disorders in cattle, sheep, and goats. For the 129 isolates analyzed, both q-PCR and IHA assays exhibited nearly complete agreement for capsular types A1 (k = 0.965) and A2 (k = 0.888) and substantial agreement for A6 (k = 0.801). Despite the overall good performance of the commercial q-PCR, its effectiveness differed between the host origin of the isolates. The serotype was identified by q-PCR in 83.3 % of cattle, 77.8 % of goat, and 53.8 % of sheep isolates. Combining the results of both methods, A1 was the most prevalent in cattle and sheep (55.6 % and 22.25 %, respectively) but was not detected in goats, A2 was the most prevalent in goats (61.1 %) and the second most prevalent in cattle (16.7 %) and sheep (20.5 %). The prevalence of A6 was 7.4 %, 5.1 %, and 16.7 % in cattle, sheep, and goats, respectively. Other capsular types determined exclusively by IHA were A16 in cattle, A9 in goats, and A7, A8, A9, and A13 in sheep. Capsular type diversity was greater in sheep (H = 0.601) than in cattle (H = 0.408) and goat (H = 0.330) isolates. The commercial multiplex q-PCR is a valuable tool, alternative to IHA, for identifying isolates of capsular types A1, A2, and A6, the most frequent serotypes of M. haemolytica associated with respiratory disease in ruminants. However, when testing sheep isolates it should be complemented with immunological assays due to the wider range of serotypes implicated.
PB Elsevier
SN 0378-1135
YR 2021
FD 2021
LK https://hdl.handle.net/20.500.14352/93339
UL https://hdl.handle.net/20.500.14352/93339
LA eng
NO Arnal, J. L., Fernández, A., Vela, A. I., Sanz, C., Fernández-Garyzábal, J. F., & Cid, D. (2021). Capsular type diversity of Mannheimia haemolytica determined by multiplex real-time PCR and indirect hemagglutination in clinical isolates from cattle, sheep, and goats in Spain. Veterinary microbiology, 258, 109121. https://doi.org/10.1016/j.vetmic.2021.109121
NO Ministerio de Ciencia e Innovación (España)
DS Docta Complutense
RD 6 abr 2025