RT Journal Article
T1 Cryopreservation of captive roe deer (Capreolus capreolus) semen
A1 Prieto-Pablos, Maria Teresa
A1 Sánchez Calabuig, María Jesús
A1 Hildebrandt, Thomas B
A1 Göritz, Fran
A1 Ortmann, Silvie
A1 Eder, S
A1 Santiago Moreno, Julian
A1 Hermes, Robert
A1 Saragusty, Joseph
AB To address the need to preserve current genetic diversity before it is lost forever; further studies to adapt assisted reproductive technologies to various endangered species are needed, among other things. Roe deer (Capreolus capreolus), an over abundant wild deer, can serve as model species to develop or improve sperm cryopreservation of threatened or endangered deer species. The aim of this study was to compare the ability of three diluents (Berliner Cryomedium [BC]; Tris, citric acid, glucose [TCG]; TES, Tris, glucose) to support chilling, cryopreservation (with 5% glycerol; G) and postthaw incubation (at 22 °C and 37 °C) of roe deer spermatozoa collected by electroejaculation. Berliner Cryomedium was the diluent that better preserved roe deer spermatozoa during refrigeration, able to maintain motility for at least 14 days, longer than the other extenders. BC + G was the extender of choice for cryopreservation, showing higher viability compared with TCG + G (66.7 ± 3.4 vs. 54.5 ± 6.5; P < 0.05) and higher level of acrosome integrity compared with TES, Tris, glucose + G (79.4 ± 3.4 vs. 67.9 ± 5.0; P < 0.05). Maintaining the samples at 22 °C after thawing presented higher values in various parameters compared with 37 °C. The knowledge gained through this study can potentially act as a preliminary step toward development of new protocols to help increase the reproductive success of biologically similar, yet endangered, wild species.
PB Elsevier
SN 0093-691X
YR 2016
FD 2016
LK https://hdl.handle.net/20.500.14352/117859
UL https://hdl.handle.net/20.500.14352/117859
LA eng
NO Prieto-Pablos, Sánchez-Calabuig, Hildebrandt, Göritz, Ortmann, Eder, Santiago-Moreno, Hermes, & Saragusty. (2016). Cryopreservation of captive roe deer (Capreolus capreolus) semen. Theriogenology, 86(3), 695-703. https://doi.org/10.1016/J.THERIOGENOLOGY.2016.02.023
NO Ministerio de Educación y Formación Profesional (España)
DS Docta Complutense
RD 6 abr 2025