RT Journal Article T1 Effect of Lachancea thermotolerans on the Formation of Polymeric Pigments during Sequential Fermentation with Schizosaccharosmyces pombe and Saccharomyces cerevisiae A1 Escott Pérez, Carlos A1 Morata, Antonio A1 Ricardo-da-Silva, Jorge A1 Callejo, María Jesús A1 González, María del Carmen A1 Suárez-Lepe, José Antonio AB Anthocyanins in red grape musts may evolve during the winemaking process and wine aging for several different reasons; colour stability and evolution is a complex process that may depend on grape variety, winemaking technology, fermentative yeast selection, co-pigmentation phenomena and polymerization. The condensation of flavanols with anthocyanins may occur either with the flavylium ion or with the hemiacetal formation in order to produce oligomers and polymers. The kinetics of the reaction are enhanced by the presence of metabolic acetaldehyde, promoting the formation of pyranoanthocyanin-type dimers or flavanol-ethyl-anthocyanin structures. The experimental design carried out using white must corrected with the addition of malvidin-3-O-glucoside and flavanols, suggests that non-Saccharomyces yeasts are able to provide increased levels of colour intensity and larger polymeric pigment ratios and polymerization indexes. The selection of non-Saccharomyces genera, in particular Lachancea thermotolerans and Schizosaccharomyces pombe in sequential fermentation, have provided experimental wines with increased fruity esters, as well as producing wines with potential pigment compositions, even though there is an important reduction of total anthocyanins. PB MDPI SN 1420-3049 YR 2018 FD 2018-09-14 LK https://hdl.handle.net/20.500.14352/113771 UL https://hdl.handle.net/20.500.14352/113771 LA eng NO Escott, C., Morata, A., Ricardo-da-Silva, J. M., Callejo, M. J., González, M. D. C., & Suarez-Lepe, J. A. (2018). Effect of Lachancea thermotolerans on the Formation of Polymeric Pigments during Sequential Fermentation with Schizosaccharosmyces pombe and Saccharomyces cerevisiae. Molecules, 23(9), 2353. https://doi.org/10.3390/molecules23092353 NO Author Contributions: Conceptualization, C.E. and A.M.; Data curation, C.E., J.M.R.-d.-S. and M.J.C.; Formal analysis, C.E. and A.M. ; Funding acquisition, A.M. and J.M.R.-d.-S.; Investigation, C.E.; Methodology, C.E.; Project administration, A.M. and J.A.S.-L.; Resources, A.M., J.M.R.-d.-S. and J.A.S.-L. ; Software, C.E.; Supervision, A.M., J.M.R.-d.-S. and J.A.S.-L.; Validation, A.M., J.M.R.-d.-S. and M.d.C.G.; Writing-original draft, C.E.; Writing-review & editing, C.E., M.J.C. and M.d.C.G. NO Ministerio de Economía, Industria y Competitividad (España) NO Fundação para a Ciência e a Tecnologia (Portugal) DS Docta Complutense RD 7 abr 2025