RT Journal Article
T1 Gray fox (Urocyon cinereoargenteus) identified as a new intermediate host for Sarcocystis neurona
A1 Dubey, Jitender P.
A1 Gupta, Aditya
A1 Calero Bernal, Rafael
A1 De Araujo, Larissa S.
A1 García-Gil, María L.
A1 Battle, Jaquin
A1 Ankarah, Ankrah
A1 Van Why, Kyle
A1 Brown, Justin D
A1 Rosenthal, Benjamin M.
AB The protozoan Sarcocystis neurona can cause severe disease in horses, marine mammals, and several other animal species in North America. The Virginia opossum (Didelphis virginiana) is its definitive host, and the raccoon (Procyon lotor) has been considered its primary intermediate host in the USA. Although canids have not previously been identified as important intermediate hosts for this parasite, we here report several natural cases in the gray fox (Urocyon cinereoargenteus). We identified muscular infections in 11 (23.9 %) of 46 gray foxes from Pennsylvania, USA. In hematoxylin and eosin (HE)-stained sections of tongue and limb muscles, only 13 sarcocysts were detected in 7 of 46 foxes, in limb muscle of 4 and in tongue of 4. In HE-stained sections, the sarcocyst wall was up to 2.7 μm-thick and contained finger-like villar protrusions. In unstained muscle squashes, 44 sarcocysts were detected; they were up to 1200 μm long and 69 μm wide. From each infected fox, only one morphologic type of sarcocyst was found. By transmission electron microscopy, these sarcocysts appeared identical to those in animals experimentally infected with S. neurona. Molecularly, sarcocysts were characterized using 18S rRNA, 28S rRNA, cox1, ITS1, rpoB, RON1, RON2, RON3, GAPDH1, ROP20, ROP21, ROP39, SnSRS21 and TUBA1; results confirmed the presence of S. neurona in the gray fox. This is the first report of muscular sarcocysts in the gray fox.
PB Elsevier
YR 2024
FD 2024
LK https://hdl.handle.net/20.500.14352/109720
UL https://hdl.handle.net/20.500.14352/109720
LA eng
NO Hernández-Camacho, N., Pineda-López, R. F., de Jesús Guerrero-Carrillo, M., Cantó-Alarcón, G. J., Jones, R. W., Moreno-Pérez, M. A., Mosqueda-Gualito, J. J., Zamora-Ledesma, S., & Camacho-Macías, B. (2016). Gray fox (Urocyon cinereoargenteus) parasite diversity in central Mexico. International journal for parasitology. Parasites and wildlife, 5(2), 207–210. https://doi.org/10.1016/j.ijppaw.2016.06.003
NO Department of Energy (DOE)( Estados Unidos)
NO Department of Agriculture (USDA) (Estados Unidos)
DS Docta Complutense
RD 6 abr 2025