RT Journal Article
T1 Magnetic beads-based electrochemical immunosensing of HIF-1α, a biomarker of tumoral hipoxia
A1 Muñoz San Martín, Cristina
A1 Gamella Carballo, María
A1 Pedrero Muñoz, María
A1 Montero Calle, Ana
A1 Barderas Manchado, Rodrigo
A1 Campuzano Ruiz, Susana
A1 Pingarrón Carrazón, José Manuel
AB Hypoxia-inducible factor-1 alpha (HIF-1α) is a transcription factor involved in tumor growth and metastasis by regulating genes involved in response to hypoxia. This work reports the first magnetic beads (MBs)-based electrochemical immunoassay for the determination of HIF-1α. The design involves a sandwich immunoassay using MBs and amperometric detection at disposable screen-printed carbon electrodes (SPCEs). Magnetic immunoconjugates of carboxylic acid-microsized magnetic particles (HOOC-MBs) modified with a specific antibody were used to selectively capture the target protein which was sandwiched with a biotinylated secondary antibody further conjugated with a streptavidin peroxidase conjugate (Strep-HRP). The great analytical performance of the immunoassay in terms of selectivity and low limit of detection (76 pg mL−1) for HIF-1α allows the determination of the endogenous amount of this biomarker in 0.5 μg raw cancer cells lysates cultured in normoxia or induced-hypoxia with CoCl2. In addition, the developed immunosensing approach was employed to analyze HIF-1α in saliva samples with results that agree with those provided by an ELISA kit involving the same immunoreagents.
PB Elsevier
YR 2020
FD 2020-03-20
LK https://hdl.handle.net/20.500.14352/113647
UL https://hdl.handle.net/20.500.14352/113647
LA eng
NO Cristina Muñoz-San Martín, Maria Gamella, María Pedrero, Ana Montero-Calle, Rodrigo Barderas, Susana Campuzano, José M. Pingarrón, Magnetic beads-based electrochemical immunosensing of HIF-1α, a biomarker of tumoral hypoxia, Sensors and Actuators B: Chemical, Volume 307, 2020, 127623, ISSN 0925-4005, https://doi.org/10.1016/j.snb.2019.127623. (https://www.sciencedirect.com/science/article/pii/S0925400519318222)
NO Ministerio de Economía y Competitividad
NO Comunidad de Madrid
NO Universidad Complutense de Madrid
NO Ministerio de Educación, Cultura y Deporte
DS Docta Complutense
RD 23 abr 2025