%0 Journal Article
%A Ojeda Fernández, Irene
%A Moreno Guzmán, María
%A González Cortés, Araceli
%A Yáñez-Sedeño Orive, Paloma
%A Pingarrón Carrazón, José Manuel
%T Electrochemical Magnetic Immunosensors for the Determination of Ceruloplasmin
%D 2013
%@ (Online )1521-4109
%U https://hdl.handle.net/20.500.14352/42743
%X Electrochemical immunosensors for ceruloplasmin (Cp) are reported for the first time. Two configurations involving magnetic beads (MBs) functionalized with Protein A or Streptavidin for immobilization of Cp antibodies were compared, using competitive immunoassay with synthesized alkaline phosphatase-Cp conjugate. Upon capturing MBs-immunoconjugates onto screen-printed carbon electrodes, quantification of Cp was accomplished by DPV measurement of 1-naphthol generated after 1-naphthylphosphate addition. Linear ranges of calibration curves and detection limits were 0.1–1000 µg/mL and 0.040 µg/mL (Protein A-MBs), and 0.025–20 µg/mL and 0.018 µg/mL (Strept-MBs). Good results were obtained in the determination of Cp in spiked human serum samples.
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