RT Journal Article
T1 Electrochemical Magnetic Immunosensors for the Determination of Ceruloplasmin
A1 Ojeda Fernández, Irene
A1 Moreno Guzmán, María
A1 González Cortés, Araceli
A1 Yáñez Sedeño, Paloma
A1 Pingarrón Carrazón, José Manuel
AB Electrochemical immunosensors for ceruloplasmin (Cp) are reported for the first time. Two configurations involving magnetic beads (MBs) functionalized with Protein A or Streptavidin for immobilization of Cp antibodies were compared, using competitive immunoassay with synthesized alkaline phosphatase-Cp conjugate. Upon capturing MBs-immunoconjugates onto screen-printed carbon electrodes, quantification of Cp was accomplished by DPV measurement of 1-naphthol generated after 1-naphthylphosphate addition. Linear ranges of calibration curves and detection limits were 0.1–1000 µg/mL and 0.040 µg/mL (Protein A-MBs), and 0.025–20 µg/mL and 0.018 µg/mL (Strept-MBs). Good results were obtained in the determination of Cp in spiked human serum samples.
PB Wiley-VCH
SN (Online )1521-4109
YR 2013
FD 2013
LK https://hdl.handle.net/20.500.14352/42743
UL https://hdl.handle.net/20.500.14352/42743
LA eng
NO Ministerio de Economía y Competitividad
NO Comunidad de Madrid
DS Docta Complutense
RD 30 abr 2024