RT Journal Article
T1 Electrochemical Magnetic Immunosensors for the Determination of Ceruloplasmin
A1 Ojeda Fernández, Irene
A1 Moreno Guzmán, María
A1 González Cortés, Araceli
A1 Yáñez-Sedeño Orive, Paloma
A1 Pingarrón Carrazón, José Manuel
AB Electrochemical immunosensors for ceruloplasmin (Cp) are reported for the first time. Two configurations involving magnetic beads (MBs) functionalized with Protein A or Streptavidin for immobilization of Cp antibodies were compared, using competitive immunoassay with synthesized alkaline phosphatase-Cp conjugate. Upon capturing MBs-immunoconjugates onto screen-printed carbon electrodes, quantification of Cp was accomplished by DPV measurement of 1-naphthol generated after 1-naphthylphosphate addition. Linear ranges of calibration curves and detection limits were 0.1–1000 µg/mL and 0.040 µg/mL (Protein A-MBs), and 0.025–20 µg/mL and 0.018 µg/mL (Strept-MBs). Good results were obtained in the determination of Cp in spiked human serum samples.
PB Wiley-VCH
SN (Online )1521-4109
YR 2013
FD 2013
LK https://hdl.handle.net/20.500.14352/42743
UL https://hdl.handle.net/20.500.14352/42743
LA eng
NO Ministerio de Economía y Competitividad
NO Comunidad de Madrid
DS Docta Complutense
RD 29 sept 2024