%0 Journal Article
%A Søndergaard, Annette
%A San Millan, Alvaro
%A Santos López, Alfonso
%A Nielsen, Signe M
%A González Zorn, Bruno
%A Nørskov-Lauritsen, Niels
%T Molecular organization of small plasmids bearing blaTEM-1 and conferring resistance to β-lactams in Haemophilus influenzae
%D 2012
%@ 1098-6596
%U https://hdl.handle.net/20.500.14352/45168
%X TEM-1 is the dominant β-lactamase of Haemophilus influenzae and can be located on small plasmids. Three distinct plasmids with sizes from 4,304 to 5,646 nucleotides (nt) were characterized: pA1606, pA1209, and pPN223. In addition to TEM-1 and a replication enzyme of the Rep 3 superfamily, pA1606 carries a Tn3 resolvase gene and pA1606 and pA1209 carry an open reading frame (ORF) similar to a plasmid recombination enzyme gene described in Gram-positive bacteria. The plasmids transformed strain Rd to the ampicillin-resistant phenotype.
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