RT Journal Article
T1 Preparation of Lipase-Coated, Stabilized, Hydrophobic Magnetic Particles for Reversible Conjugation of Biomacromolecules
A1 Marciello, Marzia
A1 Bolívar Bolívar, Juan Manuel
A1 Filice, Marco
A1 Mateo, Cesar
A1 Guisan, Jose M.
AB This Communication presents the developmentof a novel strategy for the easy conjugation of biomolecules tohydrophobic magnetic microparticles via reversible coating withpreviously functionalized lipase molecules. First, the ability oflipase to be strongly adsorbed onto hydrophobic surfaces wasexploited for the stabilization of microparticles in aqueousmedium by the creation of a hydrophilic surface. Second, thesurface amino acids of lipase can be tailored to suit biomoleculeconjugation. This approach has been demonstrated by amino-epoxy activation of lipase, enabling the conjugation of differentbiomolecules to the magnetic particle’s surface. For example, itwas possible to immobilize 70% ofEscherichia coliproteins onthe recovered particles. Furthermore, this strategy could beextended to other lipase chemical modification protocols,enablingfine control of biomolecule coupling. These conjugation techniques constitute a modular methodology that also permitsthe recycling of the magnetic carrier following use
SN 1525-7797
YR 2013
FD 2013-02-18
LK https://hdl.handle.net/20.500.14352/100852
UL https://hdl.handle.net/20.500.14352/100852
LA eng
NO Marciello M, Bolivar JM, Filice M, Mateo C, Guisan JM. Preparation of Lipase-Coated, Stabilized, Hydrophobic Magnetic Particles for Reversible Conjugation of Biomacromolecules. Biomacromolecules 2013;14:602–7. https://doi.org/10.1021/bm400032q.
NO Ministerio de Ciencia, Innovación y Universidades (España)
DS Docta Complutense
RD 8 abr 2025