RT Journal Article
T1 A new role of Klumpfuss in establishing cell fate during the GMC asymmetric cell division
A1 Gabilondo, Hugo
A1 Losada Pérez, María De La Paloma
A1 Monedero, Ignacio
A1 Torres-Herráez, Arturo
A1 Molina, Isabel
A1 Torroja, Laura
A1 Benito-Sipos, Jonathan
AB Studies in the Drosophila embryonic NB4-2 lineage have suggested that the transcription factor Klumpfuss (Klu) functions within embryonic neuroblast lineages to differentiate between the identities of two adjacent ganglion mother cells (GMCs). However, because of the limited lineage markers available, these observations have been made only for the NB4-2 lineage. Recent findings have placed this transcription factor in the vanguard of Drosophila neural stem cell biology by demonstrating that Klu is necessary for larval neuroblast growth and self-renewal. Here, we have studied the role of klu in an incipient model in order to address basic mechanisms of neural specification: the Va system. None of the previously reported roles of Klu satisfactorily explain our observations. Unexpectedly, in this lineage, klu is necessary for differentiating between the fates of the two neurons born from a unique GMC; klu mutants produce two B-type cells, rather than one B-type (Notch-OFF) and one A-type (Notch-ON) cell. Additionally, our results demonstrate that Klu operates in the GMC and/or in the newly born neuron, but not in the neuroblast. Unlike in larval neuroblasts in which Klu is an executor of Notch signaling, we have found that Klu does not lie downstream of the Notch pathway in this cell division context.
PB Springer
SN 0302-766X
YR 2014
FD 2014
LK https://hdl.handle.net/20.500.14352/94802
UL https://hdl.handle.net/20.500.14352/94802
LA eng
NO Gabilondo, H., Losada-Pérez, M., Monedero, I. et al. A new role of Klumpfuss in establishing cell fate during the GMC asymmetric cell division. Cell Tissue Res 358, 621–626 (2014). https://doi.org/10.1007/s00441-014-1965-y
NO This work was supported by a grant from the Spanish Ministerio de Ciencia e Innovación (BFU-2008-04683-C02-02) to L.T and an FPI-UAM Fellowship to H.G.
NO Ministerio de Ciencia e Innovación (España)
NO Universidad Autónoma de Madrid
DS Docta Complutense
RD 24 abr 2025