RT Journal Article
T1 Susceptibility testing of Prototheca bovis isolates from cases of bovine mastitis using the CLSI reference broth microdilution method and the Sensititre Yeast One colorimetric panel
A1 Álvarez Pérez, Sergio
A1 Anega, Blanca
A1 Diaz de Tuesta, Juan
A1 González Martín, Juan Vicente
A1 Riesgo-Martinez, Mercedes
A1 García Sánchez, Marta Eulalia
A1 Blanco Cancelo, José Luis
AB A total of 62 Prototheca bovis isolates from cases of bovine mastitis were tested for susceptibility to different antifungal compounds by the Clinical and Laboratory Standards Institute (CLSI) reference microdilution method and a commercial colorimetric microdilution panel (Sensititre YeastOne). All isolates displayed low susceptibility to echinocandins (MICs > 8 μg/ml for anidulafungin, caspofungin, and micafungin), flucytosine (MIC > 64 μg/ml), and the azoles enilconazole and fluconazole (MICs > 4 and > 64 μg/ml, respectively). Moreover, 45.2, 32.3, and 1.6% of isolates had MICs > 4 μg/ml for ketoconazole, terbinafine, and voriconazole, respectively, when tested by the CLSI method. In contrast, all isolates were more susceptible to the polyene compounds amphotericin B and nystatin, and itraconazole, posaconazole, and ravuconazole (MICs ≤ 2 μg/ml, in all cases). Comparison of the results obtained in the CLSI and Sensititre methods showed excellent essential agreement (EA) for azoles (98.4% for itraconazole and posaconazole, and 100% for voriconazole) and moderate EA for amphotericin B (72.6%), when MICs were read after 48 and 24 h of incubation, respectively. In contrast, much lower EA values were obtained in some cases when the MICs for both techniques were determined after 48 h of incubation (e.g., 9.7% for amphotericin B and 69.4% for posaconazole). Therefore, the CLSI broth microdilution method and the Sensititre YeastOne panel can be used indistinctly for susceptibility testing of P. bovis isolates against azoles but not against amphotericin B until further optimization of the test conditions.
PB Oxford Academic
YR 2021
FD 2021
LK https://hdl.handle.net/20.500.14352/122296
UL https://hdl.handle.net/20.500.14352/122296
LA eng
NO Álvarez-Pérez, S., Anega, B., Díaz-de-Tuesta, J. A., González-Martín, J. V., Riesgo-Martínez, M., García, M. E., & Blanco, J. L. (2021). Susceptibility testing of Prototheca bovis isolates from cases of bovine mastitis using the CLSI reference broth microdilution method and the Sensititre YeastOne colorimetric panel. Medical mycology, 59(12), 1257–1261. https://doi.org/10.1093/mmy/myab063
NO Contrato Ramón y Cajal RYC2018-023847-I: Sergio Álvarez Pérez
NO Ministerio de Ciencia, Innovación y Universidades (España)
DS Docta Complutense
RD 16 jul 2025