RT Journal Article
T1 Mucus interactions with liposomes encapsulating bioactives: Interfacial tensiometry and cellular uptake on Caco-2 and cocultures of Caco-2/HT29-MTX
A1 Li, Yang
A1 Arranz Gutiérrez, Elena María
A1 Guri, Anilda
A1 Corredig, Milena
AB Structuring of delivery matrices in foods aquires careful designing for optimal delivery and subsiquent absorption of the beneficial compounds in the gut. There has been quite improvement in mimicking digestion and absorption in vitro but as of yet little is understood on mucus interference in nutrient absorption Therefore in this study interactions of human intestinalmucuswith milk and soy phospholipids liposomes carring hydrophilic (epigallocatechin- 3-gallate) or hydrophobic (β-carotene) bioactive molecules were investigated. Liposomes of about 100 nm were obtained using microfluidization and their behaviour with the human intestinal mucus were evaluated using drop shape tensiometry. The chemistry of the liposomes (milk or soy) and the encapsulated bioactive structure can affect the viscoelastic behaviour of the complex itself. Empty or loaded liposomes were differently interacting with the mucus at the interface. Mucus-liposomes interactions were also studied using cell cultures, Caco-2 (without mucus) and cocultures Caco-2/HT29-MTX (mucus producing). The interaction of mucus layer with liposomes was at some extent aligned with rheological studies. This work demonstrated that delivery systems may interact with the mucosal surface of intestinal cells, and in vitro approaches allowfor screening of such interactions. These highlights could help us in carefully designing the delivery systems and moreover choosing the right carrier and/or bioactive that does not jeopardize the optimal delivery of the bioactive structure.
PB Elsevier
SN 0963-9969
YR 2017
FD 2017
LK https://hdl.handle.net/20.500.14352/93615
UL https://hdl.handle.net/20.500.14352/93615
LA eng
NO Li, Y., Arranz, E., Guri, A., & Corredig, M. (2017). Mucus interactions with liposomes encapsulating bioactives: Interfacial tensiometry and cellular uptake on Caco-2 and cocultures of Caco-2/HT29-MTX. Food research international, 92, 128-137.
NO Natural Sciences and Engineering Council of Canada
NO Fundación Alfonso Martin Escudero
DS Docta Complutense
RD 18 dic 2025