RT Journal Article
T1 An Electrochemical Enzyme Biosensor for 3-Hydroxybutyrate Detection Using Screen-Printed Electrodes Modified by Reduced Graphene Oxide and Thionine
A1 Martínez García, Gonzalo
A1 Pérez Julián, Elena
A1 Agüí Chicharro, María Lourdes
A1 Cabré, Naomí
A1 Joven, Jorge
A1 Yáñez-Sedeño Orive, Paloma
A1 Pingarrón Carrazón, José Manuel
AB A biosensor for 3-hydroxybutyrate (3-HB) involving immobilization of the enzyme 3-hydroxybutyrate dehydrogenase onto a screen-printed carbon electrode modified with reduced graphene oxide (GO) and thionine (THI) is reported here. After addition of 3-hydroxybutyrate or the sample in the presence of NAD+ cofactor, the generated NADH could be detected amperometrically at 0.0 V vs. Ag pseudo reference electrode. Under the optimized experimental conditions, a calibration plot for 3-HB was constructed showing a wide linear range between 0.010 and 0.400 mM 3-HB which covers the clinically relevant levels for diluted serum samples. In addition, a limit of detection of 1.0 µM, much lower than that reported using other biosensors, was achieved. The analytical usefulness of the developed biosensor was demonstrated via application to spiked serum samples.
PB MDPI
SN 2079-6374
YR 2017
FD 2017-11-11
LK https://hdl.handle.net/20.500.14352/19175
UL https://hdl.handle.net/20.500.14352/19175
LA eng
NO Ministerio de Economía y Competitividad (MINECO)
NO Comunidad de Madrid
DS Docta Complutense
RD 7 abr 2025