RT Journal Article
T1 Increased Levels of Anti-Anisakis Antibodies During Hospital Admission in Septic Patients
A1 Andreu-Ballester, Juan Carlos
A1 Navarro, Amparo
A1 Arribas, Miguel Angel
A1 Rico, Moises
A1 Albert, Laura
A1 García-Ballesteros, Carlos
A1 Galindo-Regal, Lorena
A1 Sorando-Serra, Rosa
A1 López-Chuliá, Francisca
A1 Peydro, Federico
A1 Rodero Martínez, Marta
A1 González Fernández, Juan
A1 Cuéllar Del Hoyo, María Del Carmen
AB Background/objectives: In a previous study, we described elevated anti-Anisakis IgG levels in septic patients in relation to disease severity. In this study, our objective was to analyze the evolution of anti-Anisakis immunoglobulins in septic patients during hospital admission and their association with αβ and γδ T cell subsets.Methods: We recruited 80 subjects: 40 patients with sepsis and 40 controls. αβ and γδ T cells were analyzed using flow cytometry. Apoptosis was also assessed, and anti-Anisakis antibodies were measured by ELISA in the sera of patients with sepsis and controls.Results: In the second analysis (7-10 after sepsis evolution), an increase in all specific antibody isotypes was identified in individuals with septic shock, except IgE. The levels of anti-Anisakis IgG and IgA were higher in the subjects with sepsis in the first analysis and continued to increase in the second analysis compared with the healthy control subjects. There was an increase in anti-Anisakis IgG and IgA levels in surviving patients and an increase in IgA levels in non-surviving patients. A rise in specific IgG and IgE levels was noted in the second analysis of patients with sepsis with αβ CD3+ T cell deficiency. Patients without γδ T cell deficiency had increased anti-Anisakis IgA levels 7-10 days after admission.Conclusions: Our results suggest a previous infection by Anisakis that could be involved in the subsequent septic process and be related to patients who have negative cultures in which the pathogen causing sepsis has not been identified.
PB MDPI
YR 2024
FD 2024-11-27
LK https://hdl.handle.net/20.500.14352/113488
UL https://hdl.handle.net/20.500.14352/113488
LA eng
NO Andreu-Ballester JC, Navarro A, Arribas MA, Rico M, Albert L, García-Ballesteros C, Galindo-Regal L, Sorando-Serra R, López-Chuliá F, Peydro F, Rodero M, González-Fernández J, Cuéllar C. Increased Levels of Anti-Anisakis Antibodies During Hospital Admission in Septic Patients. Antibodies (Basel). 2024 Nov 27;13(4):96. doi: 10.3390/antib13040096. PMID: 39727479; PMCID: PMC11672462.
DS Docta Complutense
RD 14 dic 2025