%0 Journal Article
%A Peltomaa, Riikka Johanna
%A Fikacek, Sabrina
%A Benito Peña, María Elena
%A Barderas, Rodrigo
%A Head, Trajen
%A Deo, Sapna
%A Daunert, Sylvia
%A Moreno Bondi, María Cruz
%T Bioluminescent detection of zearalenone using recombinant peptidomimetic Gaussia luciferase fusion protein
%D 2020
%@ 0026-3672
%U https://hdl.handle.net/20.500.14352/92751
%X The development of a bioluminescent immunosensor is reported for the determination of zearalenone (ZEA) based on a peptide mimetic identified by phage display. The peptide mimetic GW, with a peptide sequence GWWGPYGEIELL, was used to create recombinant fusion proteins with the bioluminescent Gaussia luciferase (GLuc) that were directly used as tracers for toxin detection in a competitive immunoassay without the need for secondary antibodies or further labeling. The bioluminescent sensor, based on protein G–coupled magnetic beads for antibody immobilization, enabled determination of ZEA with a detection limit of 4.2 ng/mL (corresponding to 420 μg/kg in food samples) and an IC50 value of 11.0 ng/mL. The sensor performance was evaluated in spiked maize and wheat samples, with recoveries ranging from 87 to 106% (RSD < 20%, n = 3). Finally, the developed method was applied to the analysis of a naturally contaminated reference matrix material and good agreement with the reported concentrations was obtained.
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