RT Journal Article
T1 Bioluminescent detection of zearalenone using recombinant peptidomimetic Gaussia luciferase fusion protein
A1 Peltomaa, Riikka Johanna
A1 Fikacek, Sabrina
A1 Benito Peña, María Elena
A1 Barderas, Rodrigo
A1 Head, Trajen
A1 Deo, Sapna
A1 Daunert, Sylvia
A1 Moreno Bondi, María Cruz
AB The development of a bioluminescent immunosensor is reported for the determination of zearalenone (ZEA) based on a peptide mimetic identified by phage display. The peptide mimetic GW, with a peptide sequence GWWGPYGEIELL, was used to create recombinant fusion proteins with the bioluminescent Gaussia luciferase (GLuc) that were directly used as tracers for toxin detection in a competitive immunoassay without the need for secondary antibodies or further labeling. The bioluminescent sensor, based on protein G–coupled magnetic beads for antibody immobilization, enabled determination of ZEA with a detection limit of 4.2 ng/mL (corresponding to 420 μg/kg in food samples) and an IC50 value of 11.0 ng/mL. The sensor performance was evaluated in spiked maize and wheat samples, with recoveries ranging from 87 to 106% (RSD < 20%, n = 3). Finally, the developed method was applied to the analysis of a naturally contaminated reference matrix material and good agreement with the reported concentrations was obtained.
PB Springer
SN 0026-3672
YR 2020
FD 2020
LK https://hdl.handle.net/20.500.14352/92751
UL https://hdl.handle.net/20.500.14352/92751
LA eng
NO Universidad Complutense de Madrid
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Foundation for the National Institutes of Health (USA)
DS Docta Complutense
RD 20 jul 2024