RT Journal Article
T1 Synthetically induced Arabidopsis thaliana autotetraploids provide insights into the analysis of meiotic mutants with altered crossover frequency
A1 Parra‐Nunez, Pablo
A1 Fernández Jiménez, Nadia
A1 Pachon‐Penalba, Miguel
A1 Sanchez‐Moran, Eugenio
A1 Pradillo Orellana, Mónica
A1 Santos Coloma, Juan Luis
AB Mutations affecting crossover (CO) frequency and distribution lead to the presence of univalents during meiosis, giving rise to aneuploid gametes and sterility. These mutations may have a different effect after chromosome doubling. The combination of altered ploidy and mutations could be potentially useful to gain new insights into the mechanisms and regulation of meiotic recombination; however, studies using autopolyploid meiotic mutants are scarce. Here, we have analyzed the cytogenetic consequences in colchicine-induced autotetraploids (colchiploids) from different Arabidopsis mutants with an altered CO frequency. We have found that there are three types of mutants: mutants in which chiasma frequency is doubled after chromosome duplication (zip4, mus81), as in the control; mutants in which polyploidy leads to a higher-than-expected increase in chiasma frequency (asy1, mer3, hei10, and mlh3); and mutants in which the rise in chiasma frequency produced by the presence of two extrachromosomal sets is less than doubled (msh5, fancm). In addition, the proportion of class I/class II COs varies after chromosome duplication in the control. The results obtained reveal the potential of colchiploid meiotic mutants for better understanding of the function of key proteins during plant meiosis. This is especially relevant considering that most crops are polyploids.
PB Wiley
SN 0028-646X
YR 2023
FD 2023
LK https://hdl.handle.net/20.500.14352/98936
UL https://hdl.handle.net/20.500.14352/98936
LA eng
NO Parra‐Nunez, Pablo, et al. «Synthetically Induced Arabidopsis Thaliana Autotetraploids Provide Insights into the Analysis of Meiotic Mutants with Altered Crossover Frequency». New Phytologist, vol. 241, n.o 1, enero de 2024, pp. 197-208. https://doi.org/10.1111/nph.19366.
NO Ministerio de Economía y Competitividad (España)
NO European Commission
NO Ministerio de Ciencia, Innovación y Universidades (España)
DS Docta Complutense
RD 9 abr 2025