%0 Journal Article %A Perez‐Gordo, Marina %A Pastor Vargas, Carlos %A Jing Lin %A Bardina, Ludmilla %A Cases, Barbara %A Ibáñez, Maria Dolores %A Vivanco Martínez, Fernando %A Cuesta‐Herranz, Javier %A Sampson, Hugh %T Epitope mapping of the major allergen from Atlantic cod in Spanish population reveals different IgE‐binding patterns %D 2013 %@ 1613-4125 %U https://hdl.handle.net/20.500.14352/94354 %X Scope: IgE-epitope mapping of allergens reveal important information about antigen components involved in allergic reactions. The peptide-based microarray immunoassay has been used to map epitopes of some food allergens. We developed a peptide microarray immunoassay to map allergenic epitopes in parvalbumin from Atlantic cod (Gad m 1), the most consumed cod species in Spain.Methods and results: Sera from 13 fish-allergic patients with specific IgE to cod parvalbumin were used. A library of overlapping peptides was synthesized, representing the primary sequence of Gad m 1. Peptides were used to analyze allergen-specific IgE antibodies in patient sera. 100% of the patients recognized one antigenic region of 15 amino acids in length in Gad m 1. This region only partially correlated with one of the three antigenic determinants of Gad c 1 (Allergen M), parvalbumin from Baltic cod (Gadus callarias). In the 3D model of the protein, this region was located on the surface of the protein.Conclusion: We have identified a relevant antigenic region in Gad m 1. This epitope could be considered as a severity marker and provides additional information to improve fish allergy diagnosis and the design of safe immunotherapeutic tools. %~