RT Journal Article
T1 Epitope mapping of the major allergen from Atlantic cod in Spanish population reveals different IgE‐binding patterns
A1 Perez‐Gordo, Marina
A1 Pastor Vargas, Carlos
A1 Jing Lin, 
A1 Bardina, Ludmilla
A1 Cases, Barbara
A1 Ibáñez, Maria Dolores
A1 Vivanco Martínez, Fernando
A1 Cuesta‐Herranz, Javier
A1 Sampson, Hugh
AB Scope: IgE-epitope mapping of allergens reveal important information about antigen components involved in allergic reactions. The peptide-based microarray immunoassay has been used to map epitopes of some food allergens. We developed a peptide microarray immunoassay to map allergenic epitopes in parvalbumin from Atlantic cod (Gad m 1), the most consumed cod species in Spain.Methods and results: Sera from 13 fish-allergic patients with specific IgE to cod parvalbumin were used. A library of overlapping peptides was synthesized, representing the primary sequence of Gad m 1. Peptides were used to analyze allergen-specific IgE antibodies in patient sera. 100% of the patients recognized one antigenic region of 15 amino acids in length in Gad m 1. This region only partially correlated with one of the three antigenic determinants of Gad c 1 (Allergen M), parvalbumin from Baltic cod (Gadus callarias). In the 3D model of the protein, this region was located on the surface of the protein.Conclusion: We have identified a relevant antigenic region in Gad m 1. This epitope could be considered as a severity marker and provides additional information to improve fish allergy diagnosis and the design of safe immunotherapeutic tools.
PB Wiley
SN 1613-4125
YR 2013
FD 2013
LK https://hdl.handle.net/20.500.14352/94354
UL https://hdl.handle.net/20.500.14352/94354
LA eng
NO Perez-Gordo M, Pastor-Vargas C, Lin J, Bardina L, Cases B, Ibáñez MD, Vivanco F, Cuesta-Herranz J, Sampson HA. Epitope mapping of the major allergen from Atlantic cod in Spanish population reveals different IgE-binding patterns. Mol Nutr Food Res. 2013 Jul;57(7):1283-90. doi: 10.1002/mnfr.201200332. Epub 2013 Apr 2. PMID: 23554100.
NO Ministerio de Educación y  Ciencia (España)
DS Docta Complutense
RD 6 oct 2024