RT Journal Article
T1 Surface-enhanced Raman scattering-based detection of the interactions between the essential cell division FtsZ protein and bacterial membrane elements
A1 Ahijado Guzmán, Rubén
A1 Gómez-Puertas, Paulino
A1 Álvarez-Puebla, Ramón A
A1 Rivas, Germán
A1 Liz-Marzán, Luis
AB Surface-enhanced Raman scattering (SERS) spectroscopy has been applied to detect the interaction of the FtsZ protein from Escherichia coli, an essential component of the bacterial division machinery, with either a soluble variant of the ZipA protein (that provides membrane tethering to FtsZ) or the bacterial membrane (containing the full-length ZipA naturally incorporated), on silver-coated polystyrene micrometer-sized beads. The engineered microbeads were used not only to support the bilayers but also to offer a stable support with a high density of SERS hot spots, allowing the detection of ZipA structural changes linked to the binding of FtsZ. These changes were different upon incubating the coated beads with FtsZ polymers (GTP form) as compared to oligomers (GDP form) and more pronounced when the plasmonic sensors were coated with natural bacterial membranes.
SN 1936-086X
YR 2012
FD 2012
LK https://hdl.handle.net/20.500.14352/92241
UL https://hdl.handle.net/20.500.14352/92241
LA eng
NO Ahijado-Guzmán, Rubén, et al. «Surface-Enhanced Raman Scattering-Based Detection of the Interactions between the Essential Cell Division FtsZ Protein and Bacterial Membrane Elements». ACS Nano, vol. 6, n.o 8, agosto de 2012, pp. 7514-20. https://doi.org/10.1021/nn302825u.
DS Docta Complutense
RD 9 abr 2025