RT Journal Article
T1 Macroporous Calcium Phosphate/Chitosan Composites Prepared via Unidirectional Ice Segregation and Subsequent Freeze-Drying
A1 Aranaz, Inmaculada
A1 Martínez Campos, Enrique
A1 Moreno Vicente, Carolina
A1 Civantos Fernández, Ana Fátima
A1 García Arguelles, Sara
A1 del Monte, Francisco
AB Calcium phosphate chitosan-based composites have gained much interest in recent years for biomedical purposes. In this paper, three-dimensional calcium phosphate chitosan-based composites with different mineral contents were produced using a green method called ice segregation induced self-assembly (ISISA). In this methodology, ice crystals were used as a template to produce porous structures from an aqueous solution of chitosan (CS) and hydroxyapatite (Hap) also containing acetic acid (pH = 4.5). For better characterization of the nature of the inorganic matter entrapped within the resulting composite, we performed either oxygen plasma or calcination processes to remove the organic matter. The nature of the phosphate salts was studied by XRD and NMR studies. Amorphous calcium phosphate (ACP) was identified as the mineral phase in the composites submitted to oxygen plasma, whereas crystalline Hap was obtained after calcination. SEM microscopy revealed the formation of porous structures (porosity around 80–85%) in the original composites, as well as in the inorganic matrices obtained after calcination, with porous channels of up to 50 µm in diameter in the former case and of up to 20 µm in the latter. The biocompatibility of the composites was assessed using two different cell lines: C2C12GFP premyoblastic cells and MC3T3 preosteoblastic cells.
SN 1996-1944
YR 2017
FD 2017
LK https://hdl.handle.net/20.500.14352/19234
UL https://hdl.handle.net/20.500.14352/19234
LA eng
NO Ministerio de Economía y Competitividad (MINECO)
DS Docta Complutense
RD 7 may 2024