RT Journal Article
T1 Poly Lactic-co-Glycolic Acid (PLGA) Loaded with a Squaraine Dye as Photosensitizer for Antimicrobial Photodynamic Therapy
A1 Melese Dereje, Degnet
A1 Pontremoli, Carlotta
A1 García Fontecha, Ana
A1 Galliano, Simone
A1 Colilla Nieto, Montserrat
A1 González Ortiz, Blanca
A1 Vallet Regí, María Dulce Nombre
A1 Izquierdo Barba, Isabel
A1 Barbero, Nadia
AB Antimicrobial Photodynamic Therapy (aPDT) is an innovative and promising method for combating infections, reducing the risk of antimicrobial resistance compared to traditional antibiotics. Squaraine (SQ) dyes can be considered promising photosensitizers (PSs) but are generally hydrophobic molecules that can self-aggregate under physiological conditions. To overcome these drawbacks, a possible solution is to incorporate SQs inside nanoparticles (NPs). The present work deals with the design and development of innovative nanophotosensitizers based on poly lactic-co-glycolic acid (PLGA) NPs incorporating a brominated squaraine (BrSQ) with potential application in aPDT. Two designs of experiments (DoEs) based on the single emulsion and nanoprecipitation methods wereset up to investigate how different variables (type of solvent, solvent ratio, concentration of PLGA, stabilizer and dye, sonication power and time) can affect the size, zeta (ζ)-potential, yield, entrapment efficiency, and drug loading capacity of the SQ-PLGA NPs. SQ-PLGA NPs were characterized by NTA, FE-SEM, and UV-Vis spectroscopy and the ability to produce reactive oxygen species (ROS)was evaluated, proving that ROS generation ability is preserved in SQ-PLGA. In vitro antimicrobial activity against Gram-positive bacteria in planktonic state using Staphylococcus aureus was conducted in different conditions and pH to evaluate the potential of these nanophotosensitizers for aPDT in the local treatment of infections.
YR 2024
FD 2024
LK https://hdl.handle.net/20.500.14352/106821
UL https://hdl.handle.net/20.500.14352/106821
LA eng
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Universidad de Torino
NO Fundación Ramón Areces
NO European Research Council
DS Docta Complutense
RD 6 sept 2025