RT Journal Article
T1 Valorisation of the green waste parts from large-leaved buttercup (Ranunculus macrophyllus Desf.): phenolic profile and health promoting effects study
A1 Deghima, Amirouche
A1 Righi, Nadjat
A1 Rosales Conrado, Noelia
A1 León González, María Eugenia De
A1 Baali, Faiza
A1 Gómez Mejía, Esther
A1 Madrid Albarrán, María Yolanda
A1 Bedjou, Fatiha
A2 Springer, 
AB Due to the extensive use of Ranunculus macrophyllus Desf. roots for medicinal purposes, most of the leafy green parts are just wasted. The aim of this work is to valorize the leafy green parts and promote their application in different modern industries.MethodsFor this purpose, we studied the phenolic profile of R. macrophyllus Desf. (RM-B) using chromatographic and spectrophotometric methods and we tested the in-vitro antioxidant activity and the in-vivo effect of RM-B on plasma and liver antioxidant statuts.ResultsRM-B contained high amounts of polyphenols (675 mg GAE/100 g dry weigh dw) and flavonoids (105 mg QE/100 g dw). In-vitro, RM-B exhibited promising radical scavenging activity against 2,2′-azino-bis(3-éthylbenzothiazoline-6-sulphonique) (ABTS+·) (IC50: 247 µg/mL), hydrogen peroxide radicals (IC50: 626 µg/mL) and inhibited oxidative red blood cells hemolysis (IC50: 120 µg/mL), RM-B also showed strong reducing power (982 µM FeSO4/mg extract). In-vivo, RM-B improved the radical scavenging ability and reducing power of plasma and enhanced liver antioxidant status by increasing catalase and reduced glutathione levels and decreasing malondialdhyde levels without altering the key serum biochemical parameters reflecting liver and kidney functions. Polyphenols identified using capillary LC-DAD and LC–MS/MS analyses like hesperidin (131.2 mg/100 g dw), rutin (29.0 mg/100 g dw) and p-coumaric acid (5.8 mg/100 g dw), may be responsible for the health promoting effects of RM-B.ConclusionWe may conclude that R. macrophyllus Desf. is a good source of beneficial polyphenols with strong antioxidant, anti-hemolytic and health-promoting effects, which promotes its use in pharmaceutical, medicinal and nutraceutical industries.
PB Springer
SN 1877-2641
YR 2020
FD 2020
LK https://hdl.handle.net/20.500.14352/92473
UL https://hdl.handle.net/20.500.14352/92473
LA eng
NO Deghima, A; Righi, N; Rosales-Conrado, N; Leon-Gonzalez, ME; Baali, F; Gomez-Mejia, E; Madrid, Y Bedjou, F, Valorisation of the Green Waste Parts from Large-Leaved Buttercup (Ranunculus macrophyllus Desf.): Phenolic Profile and Health Promoting Effects Study. 2020,  12(8):4307-4318
NO Comunidad de Madrid
NO Ministerio de Economía y Competitividad (España)
NO European Commission
DS Docta Complutense
RD 23 jul 2024