RT Journal Article
T1 The “steric-like” inhibitory effect and mechanism of doxycycline on florfenicol metabolism: Interaction risk.
A1 Xu, X
A1 Liu, Y
A1 Guo, M
A1 Martínez Caballero, María Aranzazu
A1 Ares Lombán, Irma
A1 López Torres, Bernardo
A1 Martínez Larrañaga, María Rosa
A1 Wang, X
A1 Anadón Navarro, Arturo Ramón
A1 Martínez Caballero, Marta
AB Most of the studies on doxycycline (DOX) and florfenicol (FF) remain focused on the improvement of antimicrobial activity and antimicrobial spectrum, and there is no relevant report on whether there is interaction between the two drugs after the combination. This research study evaluated the effect of DOX on FF metabolism in vitro and its mechanisms. The findings of this study showed that DOX inhibits FF metabolism in two ways. Firstly, DOX significantly inhibits the expression of CYP3A29, leading to the slower metabolism of FF; secondly, DOX affects the binding of FF to R106 and R372 by competing for the R372 and/or by a "steric-like effect", thus slowing down FF metabolism, which may increase the residual concentration of FF in edible tissues of food producing animals.
PB Elsevier
SN 0278-6915
YR 2022
FD 2022
LK https://hdl.handle.net/20.500.14352/138654
UL https://hdl.handle.net/20.500.14352/138654
LA eng
NO Xu, X., Liu, Y., Guo, M., Martínez, M. A., Ares, I., Lopez-Torres, B., Martínez-Larrañaga, M. R., Wang, X., Anadón, A., & Martínez, M. (2022). The "steric-like" inhibitory effect and mechanism of doxycycline on florfenicol metabolism: Interaction risk. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 169, 113431. https://doi.org/10.1016/j.fct.2022.113431
NO Credit authorship contribution statementXiaoqing Xu: Conceptualization, Funding acquisition, Methodology, Validation, Formal analysis, Investigation, Writing – original draft, Writing – review & editing. Yanan Liu: Investigation, Methodology, Formal analysis, Writing original draft, Writing – review & editing. Mingyue Guo: Investigation, Formal analysis, Writing – review & editing. María-Aránzazu Martínez: Investigation, Formal analysis, Writing – review & editing. Irma Ares: Investigation, Formal analysis, Writing – review & editing. Bernardo Lopez-Torres: Investigation, Formal analysis, Writing – review & editing. Xu Wang: Conceptualization, Funding acquisition, Methodology, Validation, Formal analysis, Investigation. Arturo Anadón: Conceptualization, Funding acquisition, Methodology, Valida-tion, Formal analysis, Investigation. Marta Martínez: Conceptualization, Methodology, Investigation, Formal analysis, Writing – review & editing, Conceptualization, Funding acquisition, Methodology, Valida-tion, Formal analysis, Investigation.
NO National Key Research and Development Program of China
NO Ministerio de Ciencia, Innovación y Universidades ( España)
DS Docta Complutense
RD 20 ago 2026