RT Journal Article
T1 State of art of micronuclei assay in exfoliative cytology as a clinical biomarker of genetic damage in oral carcinogenesis: a systematic review and meta-analysis
A1 Alberto Caponio, Vito Carlo
A1 França-Vieira e Silva, Fabio
A1 Popolo, Francesco
A1 Giugliano, Sara
A1 Spizzirri, Francesca
A1 Lorenzo-Pouso, Alejandro I.
A1 Padín-Iruegas, María Elena
A1 Zhurakivska, Khrystyna
A1 Lo Muzio, Lorenzo
A1 López-Pintor Muñoz, Rosa María
AB Oral squamous cell carcinoma (OSCC) is the most common oral malignancy, often preceded by oral potentially malignant disorders (OPMDs). Currently, no clinical biomarker exists to predict malignancy, necessitating OPMD follow-up. Habits and environmental factors, such as smoking, and alcohol consumption, influence OSCC onset. Increased micronuclei (MNs) formation has been observed in the development of OSCC. Non-invasive diagnostic tests like exfoliative cytology offer painless and regular monitoring options. This study evaluates the impact of tobacco, alcohol, and pesticide exposure on MNs occurrence in exfoliative cytology-collected oral mucosal cells, assessing their potential as non-invasive biomarker for OSCC development prediction and monitoring in high-risk patients. Despite results from this meta-analysis supporting the existence of a stepwise increase from controls to patients with OPMD to OSCC, the translation of these findings into clinical practice is limited due to intra- and inter-individual heterogeneity, as well as methodological variability in MNs quantification. Various factors contribute to this heterogeneity, including demographic variables, methodological variability of different laboratories, staining techniques, sample collection location, and patient characteristics. All these points were discussed to provide further insights and improve standardization for future studies.
PB Elsevier
SN 1383-5742
YR 2024
FD 2024-07-02
LK https://hdl.handle.net/20.500.14352/107941
UL https://hdl.handle.net/20.500.14352/107941
LA eng
NO Caponio VCA, Silva FFE, Popolo F, Giugliano S, Spizzirri F, Lorenzo-Pouso AI, Padín-Iruegas ME, Zhurakivska K, Muzio LL, López-Pintor RM. State of art of micronuclei assay in exfoliative cytology as a clinical biomarker of genetic damage in oral carcinogenesis: A systematic review and meta-analysis. Mutat Res Rev Mutat Res. 2024 Jul 2;794:108508. doi: 10.1016/j.mrrev.2024.108508
DS Docta Complutense
RD 26 abr 2025