RT Journal Article
T1 Connection between miRNA Mediation and the Bioactive Effects of Broccoli (Brassica oleracea var. italica): Exogenous miRNA Resistance to Food Processing and GI Digestion
A1 Chapado, Luis A.
A1 Ruíz-Roso Guerra, María de Belén
A1 Dávalos, Alberto
AB Broccoli (Brassica oleracea var. italica) and its bioactive compounds are associated with beneficial health effects, which might be enabled, at least in part, through miRNA regulation, despite recent controversial studies suggesting that exogenous dietary miRNAs may reach host circulation and target cells to regulate gene expression. Here, a computational analysis was performed to explore the processes and pathways associated with genes targeted either by (1) host-expressed miRNAs (endogenous) modulated by the bioactive compounds in broccoli or (2) miRNAs derived from broccoli (exogenous). In addition, the stability of exogenous miRNAs from broccoli was assessed after broccoli was subjected to the usual processing methods and in vitro digestion-simulating gastrointestinal (GI) conditions. Overall, bioinformatic results show that the anticarcinogenic and cancer-preventive properties attributed to cruciferous vegetables might be mediated, at least in part, through miRNA-related mechanisms. Moreover, results show that broccoli-derived miRNAs can survive common food-processing conditions and GI digestion.
PB American Chemical Society
SN 0021-8561
YR 2021
FD 2021-08
LK https://hdl.handle.net/20.500.14352/132174
UL https://hdl.handle.net/20.500.14352/132174
LA eng
NO Chapado LA, Martín-Hernández R, Hernández de la Red S, Tomé-Carneiro J, Gil-Zamorano J, Ruiz-Roso MB, Del Saz A, Crespo MC, Del Pozo-Acebo L, Arantes Ferreira Peres W, de la Peña G, López de Las Hazas MC, Dávalos A. Connection between miRNA Mediation and the Bioactive Effects of Broccoli (Brassica oleracea var. italica): Exogenous miRNA Resistance to Food Processing and GI Digestion. J Agric Food Chem. 2021 Aug 18;69(32):9326-9337. doi: 10.1021/acs.jafc.1c04087. Epub 2021 Aug 6. PMID: 34358423.
NO Financiado con Fondos FEDER
NO European Commission
NO Ministerio de Ciencia e Innovación (España)
DS Docta Complutense
RD 23 mar 2026