RT Journal Article
T1 Growth hormone upregulates intestinal trefoil factor expression in the ileum of rats after γ-radiation
A1 Posadas, Sinforiano
A1 Largo, Carlota
A1 Merino Martín, José Joaquín
A1 Elvira, Marcos
A1 Gonzalez, Gonzalo
A1 Caz, Victor
A1 Caballero, Isabel
A1 De Miguel. Enrique, 
AB Growth hormone (GH) and intestinal trefoil factor (ITF) have been involved in intestinal protection and repair. This study investigates the effects of GH administration on ITF expression and histological changes associated with tissue injury in an intestinal rat model of radiation. Adult male rats were divided into four groups: control, GH, radiation and radiation + GH (GHyRAD). Ileum samples were obtained at 2 or 72 h after radiation and processed to determine ITF levels (mRNA and protein) by quantitative polymerase chain reaction, Western blot and immunohistochemistry. In addition, goblet ITF-positive cells were identified by immunohistochemistry at 72 h. Our results showed an upregulation of mRNA and protein production of ITF in ileum samples after GH and radiation + GH compared with control and irradiated samples. Irradiation alone affected ITF protein expression. However, irradiation after GH pretreatment produced the highest ITF mRNA and protein levels at both the tested time points. ITF-producing goblet cells were identified in intestinal villi (apical location). GH treatment increased the number of ITF-producing goblet cells, and radiation after GH treatment displayed further increase in the number of ITF-positive goblet cells. GH upregulates ITF in normal intestinal tissue. This upregulation is higher when radiation is given after GH treatment. Nevertheless, the mechanism by which GH regulates ITF expression remains unclear and is still under investigation. These results could open up new avenues in the therapeutic reparative and protective effects of GH during radiotherapy and chemotherapy.
PB SAGE Journals
SN 1535-3702
YR 2011
FD 2011
LK https://hdl.handle.net/20.500.14352/116390
UL https://hdl.handle.net/20.500.14352/116390
LA eng
NO Posadas SJ, Largo C, Merino JJ, Elvira M, Gonzalez G, Caz V, Caballero I, De Miguel E. Growth hormone upregulates intestinal trefoil factor expression in the ileum of rats after γ-radiation. Exp Biol Med (Maywood). 2011 Feb;236(2):205-11. doi: 10.1258/ebm.2010.009358. PMID: 21321317.
NO Ministerio de Sanidad (España)
DS Docta Complutense
RD 18 abr 2025