RT Journal Article
T1 Elastic remodeling of model and cell membranes by rotating ATP synthase
A1 Valdivieso González, David
A1 Sacristán Fernández, Miguel Ángel
A1 Natale, Paolo
A1 Orgaz Martín, Belén
A1 Lillo, Pilar M.
A1 Almendro Vedia, Víctor Galileo
A1 López Montero, Iván
AB Energy homeostasis in cells relies on the rotary motion of ATP synthase. The spinning movement is also assumed to impact the mechanical properties of the surrounding lipids, leading to emergent effects such as non-equilibrium membrane fluctuations or protein curvature sorting in model systems. Here, we demonstrate that ATP synthase rotation reduces lateral pressure by decreasing lipid packing in both artificial and bacterial membranes. Using micropipette aspiration and fluorescence lifetime imaging microscopy, we find that the rotation of ATP synthase lowers the membrane tension of giant unilamellar vesicles, making the membranes more flexible. Fluorescent probes sensitive to lipid packing confirm that ATP synthase lowers membrane surface pressure during rotation. In vivo experiments in Bacillus subtilis further reveal that stimulation of ATP synthase rotation with specific drugs decreases the lateral pressure of the plasma membrane. These findings suggest that ATP synthase plays a significant role in modulating the mechanical properties of native membranes, potentially driving biological processes linked to membrane dynamics and protein self-assembly.
PB Elsevier
YR 2025
FD 2025
LK https://hdl.handle.net/20.500.14352/121785
UL https://hdl.handle.net/20.500.14352/121785
LA eng
NO Valdivieso González, D., Sacristán, M. A., Natale, P., Orgaz, B., Lillo, M. P., Almendro-Vedia, V., & López-Montero, I. (2025). Elastic remodeling of model and cell membranes by rotating ATP synthase. Cell Reports Physical Science, 6(5), 102567. https://doi.org/10.1016/j.xcrp.2025.102567
NO Contribución de autores:I.L.-M. conceived the original idea, designed the research, and acquired the funding; D.V.G. conducted the experiments; D.V.G., M.A.S., M.P.L., V.A.-V., and I.L.-M. analyzed data; B.O. and P.N. provided reagents and protocols; D.V.G. and I.L.-M. wrote the paper, and all the authors reviewed and edited the manuscript.CT103/19/PEJ-2019-AI/IND-13687 CT58/21-CT59/21
NO European Commission
NO Universidad Complutense de Madrid
NO Comunidad Autónoma de Madrid
DS Docta Complutense
RD 27 jun 2025