RT Journal Article
T1 Immunologic responses to the major allergen of Olea Europaea in local and systemic allergic rhinitis subjects
A1 Campo, Paloma
A1 Villalba Díaz, Mayte
A1 Barrionuevo, Esther
A1 Rondón, Carmen
A1 Galindo, Luisa
A1 Rodríguez, María José
A1 López Rodríguez, Juan Carlos
A1 Torres, María José
A1 Blanca, Miguel
A1 Mayorga, Cristobalina
AB Evaluate: the in vivo and in vitro responses to nOle e 1 in allergic rhinitis (AR) and local allergic rhinitis (LAR) patients sensitized to olive tree pollen (OL) confirmed by nasal allergen provocation test (NAPT).Methods: Twelve subjects with AR, 12 with LAR, and 12 subjects as control group (CG) were selected. Skin testing and NAPT with nOle e 1 were performed. ECP and tryptase were measured in nasal lavages before and after NAPT. Serum IgE to OL allergens were measured by ELISA. Basophil activation tests (BAT) with OL and nOle e 1 and dendritic cell maturation/proliferation studies were carried out. Results: All AR (12/12) and 10/12 (83%) of LAR had a +NAPT to nOle e 1. ECP levels in nasal lavages were significantly increased after NAPT in both AR and LAR compared with CG at 15 minutes (p<0.05). Serum IgE was positive only in AR. All AR had +BAT responses to OL and 10/12 to nOle e 1 (83%); 8/12 LAR (66.6%) had a +BAT with OL and 4/12 (33%) to nOle e 1, with only one subject of the control group with a +BAT to both OL and nOle e 1 (8%). Dendritic cell proliferation to nOle e 1 was increased in AR compared to LAR and CG (p=0.019 and p=0.001 respectively).Conclusion: Both AR and LAR had a similar in vivo response to nOle e 1 with release of inflammatory mediators. Specific basophil activation with OL and nOle e 1 was observed in LAR confirming previous data obtained with dust mites.
PB BioMed Central
SN 2045-7022
YR 2015
FD 2015
LK https://hdl.handle.net/20.500.14352/34857
UL https://hdl.handle.net/20.500.14352/34857
LA eng
NO Spanish Health Ministry Fund for Health in Spain (FIS)
NO Ministry of Innovation
NO Andalusian Health Ministry
DS Docta Complutense
RD 28 jul 2024