RT Journal Article
T1 Morphological and molecular characterization of a new ciliate Nyctotheroides grimi n.sp. (Armophorea, Clevelandellida) from Chinese frogs
A1 Ponce Gordo, Francisco
A1 Li, M.
A1 Hu, G.
A1 Li, C.
A1 Zhao, W.S.
A1 Zou, H.
A1 Li, W.X.
A1 Wu, S.G.
A1 Wang, G.T.
AB A new species of clevelandellid ciliate, Nyctotheroides grimi n. sp., is described from the frog Fejervarya limnocharis. Light and scanning electron microscopy were used for the morphological studies, and the DNA encoding the SSU rRNA gene (SSU rDNA) and the ITS1-5.8S subunit rRNA-ITS2 region (ITS) were sequenced for genetic comparisons and phylogenetic analysis. The main distinctive morphological feature is a knob-like projection in the left-posterior end; other differential characters are the cell size, the length of the oral groove and the shape of the infundibulum. Nyctotheroides grimi possess an apical suture line in the left and right side of the anterior end and in the left side of the caudal end. In the phylogenetic analyses, the new species engroups with other Nyctotheroides species forming a monophyletic group. The high similarity in the SSU rDNA and ITS sequences between Nyctotheroides species suggests a relative recent divergence. The genetic data and the different host range support the separation of Nyctotheroides and Nyctotherus; however the morphological criterion based on the presence (in Nyctotheroides)/absence (in Nyctothterus) of an apical kinetal suture line should be modified to consider the presence of kinetal suture lines in the apical and/or the caudal left side in Nyctotheroides.
PB Elsevier
YR 2020
FD 2020
LK https://hdl.handle.net/20.500.14352/114653
UL https://hdl.handle.net/20.500.14352/114653
LA eng
NO Li M, Hu G, Li C, Zhao W, Zou H, Li W, et al. Morphological and molecular characterization of a new ciliate Nyctotheroides grimi n. sp. (Armophorea, Clevelandellida) from Chinese frogs. Acta Tropica 2020;208:105531. https://doi.org/10.1016/j.actatropica.2020.105531.
NO National Natural Science Foundation of China
NO Youth Innovation Promotion Association CAS
NO China Agriculture Research System
DS Docta Complutense
RD 11 oct 2025