RT Journal Article
T1 Rhodamine-based sensor for real-time imaging of mitochondrial ATP in living fibroblasts
A1 de la Fuente Herreruela, Diego
A1 Gónzalez Charro, Vicente
A1 Almendro Vedia, Víctor Galileo
A1 Morán, María
A1 Martín, Miguel Ángel
A1 Lillo, M. Pilar
A1 Natale, Paolo
A1 López-Montero, Iván
AB Mitochondria are essential for the production and maintenance of ATP in the eukaryotic cell. To image and monitor intracellular ATP level without cell breakage, biological and chemical sensors were developed in the last years. Here, we have internalized a rhodamine-based sensor RSL+ into living cells and monitored the mitochondrial ATP levels in cultured mouse embryonic fibroblasts. To evaluate the robustness of the sensor we imaged the changes of the mitochondrial ATP levels under non-physiological conditions upon incubation with FCCP, oligomycin, azide, deoxyglucose or phosphoenolpyruvate; all compounds that interfere with ATP homeostasis of the cell. The ATP sensor allowed us to determine the mitochondrial ATP levels in human skin fibroblasts where we observe a similar amount of ATP compared to mouse embryonic fibroblasts. We propose the RSL+ to be a valuable tool for the assessment of mitochondrial dysfunction in human cells derived from mitochondrial OXPHOS patients and for basic studies on bioenergetics metabolism.
PB Elsevier
SN 0005-2728
YR 2017
FD 2017
LK https://hdl.handle.net/20.500.14352/19028
UL https://hdl.handle.net/20.500.14352/19028
LA eng
NO The research leading to these results has received funding from the European Research Council under the European Union's Seventh Framework Programme (ERC grant agreement n° 338133)
NO Unión Europea. FP7
NO Ministerio de Economía y Competitividad (MINECO)
NO Ministerio de Economía y Competitividad (MINECO)/Instituto de Salud Carlos III (ISCIII)-FEDER
DS Docta Complutense
RD 7 abr 2025