RT Journal Article
T1 Cilastatin as a Potential Anti-Inflammatory and Neuroprotective Treatment in the Management of Glaucoma
A1 Martínez López, Miguel Ángel
A1 Rubio Casado, Sara
A1 San Felipe Riba, Diego
A1 Martín Sánchez, Beatriz
A1 Fernández Albarral, José
A1 García Martín, Elena Salobrar
A1 Matamoros, José Antonio
A1 Ramírez Sebastián, José Manuel
A1 Hoz Montañana, María Rosa De
A1 Salazar Corral, Juan José
A1 Marco López, Eva María
A1 Lázaro Fernández, Alberto
A1 López Gallardo, Meritxell
A1 Ramírez Sebastián, Ana Isabel
AB Glaucoma is a neurodegenerative disease that causes blindness. In this study, we aimed to evaluate the protective role of cilastatin (CIL), generally used in the treatment of nephropathologies associated with inflammation, in an experimental mouse model based on unilateral (left) laser-induced ocular hypertension (OHT). Male Swiss mice were administered CIL daily (300 mg/kg, i.p.) two days before OHT surgery until sacrifice 3 or 7 days later. Intraocular Pressure (IOP), as well as retinal ganglion cell (RGC) survival, was registered, and the inflammatory responses of macroglial and microglial cells were studied via immunohistochemical techniques. Results from OHT eyes were compared to normotensive contralateral (CONTRA) and naïve control eyes considering nine retinal areas and all retinal layers. OHT successfully increased IOP values in OHT eyes but not in CONTRA eyes; CIL did not affect IOP values. Surgery induced a higher loss of RGCs in OHT eyes than in CONTRA eyes, while CIL attenuated this loss. Similarly, surgery increased macroglial and microglial activation in OHT eyes and to a lesser extent in CONTRA eyes; CIL prevented both macroglial and microglial activation in OHT and CONTRA eyes. Therefore, CIL arises as a potential effective strategy to reduce OHT-associated damage in the retina of experimental mice.
PB MDPI
YR 2024
FD 2024-03-07
LK https://hdl.handle.net/20.500.14352/102168
UL https://hdl.handle.net/20.500.14352/102168
LA eng
NO Martínez-López, M.A.; Rubio-Casado, S.; San Felipe, D.; Martín-Sánchez, B.; Fernández-Albarral, J.A.; Salobrar-García, E.; Matamoros, J.A.; Ramírez, J.M.; de Hoz, R.; Salazar, J.J.; et al. Cilastatin as a Potential Anti-Inflammatory and Neuroprotective Treatment in the Management of Glaucoma. Int. J. Mol. Sci. 2024, 25, 3115. https://doi.org/10.3390/ijms25063115
NO Submission received: 10 January 2024 / Revised: 26 February 2024 / Accepted: 5 March 2024 / Published: 7 March 2024(This article belongs to the Special Issue Advanced Research in Retina 2.0)
NO Universidad Complutense deMadrid (España)
NO Ministerio de Ciencia, Innovación y Universidades de España
NO Instituto de Salud Carlos III (España)
NO Fondo Europeo de Desarrollo Regional (FEDER)
NO Comisión Europea
NO Comunidad de Madrid (España)
DS Docta Complutense
RD 21 abr 2025