RT Journal Article
T1 Isopeptide bonding in planta allows functionalization of elongated flexuous proteinaceous viral nanoparticles, including non-viable constructs by other means
A1 Truchado Martín, Daniel Alejandro
A1 Rincón, Sara
A1 Zurita, Lucía
A1 Sánchez, Flora
A1 Ponz, Fernando
A2 Guo, Huichen
A2 Abdullah, SahibzadaWaheed
AB Plant viral nanoparticles (VNPs) have become an attractive platform for the development of novel nanotools in the last years because of their safety, inexpensive production, and straightforward functionalization. Turnip mosaic virus (TuMV) is one example of a plant-based VNP used as a nanobiotechnological platform either as virions or as virus-like particles (VLPs). Their functionalization mainly consists of coating their surface with the molecules of interest via chemical conjugation or genetic fusion. However, because of their limitations, these two methods sometimes result in non-viable constructs. In this paper, we applied the SpyTag/SpyCatcher technology as an alternative for the functionalization of TuMV VLPs with peptides and proteins. We chose as molecules of interest the green fluorescent protein (GFP) because of its good traceability, as well as the vasoactive intestinal peptide (VIP), given the previous unsuccessful attempts to functionalize TuMV VNPs by other methods. The successful conjugation of VLPs to GFP and VIP using SpyTag/SpyCatcher was confirmed through Western blot and electron microscopy. Moreover, the isopeptide bond between SpyTag and SpyCatcher occurred in vivo in co-agroinfiltrated Nicotiana benthamiana plants. These results demonstrated that SpyTag/SpyCatcher improves TuMV functionalization compared with previous approaches, thus implying the expansion of the application of the technology to elongated flexuous VNPs.
PB MDPI
SN 1999-4915
YR 2023
FD 2023-01-28
LK https://hdl.handle.net/20.500.14352/102837
UL https://hdl.handle.net/20.500.14352/102837
LA eng
NO Truchado, D. A., Rincón, S., Zurita, L., Sánchez, F., & Ponz, F. (2023). Isopeptide Bonding In Planta Allows Functionalization of Elongated Flexuous Proteinaceous Viral Nanoparticles, including Non-Viable Constructs by Other Means. Viruses, 15(2), 375.
NO Unión Europea
NO Ministerio de Universidades (España)
NO Comunidad de Madrid
NO Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)
NO Universidad Complutense de Madrid (UCM)
DS Docta Complutense
RD 8 abr 2025