RT Journal Article
T1 First report and molecular characterization of cases of natural Taylorella asinigenitalis infection in three donkey breeds in Spain
A1 Dorrego Rodríguez, Abel
A1 Herranz Benito, Carmen
A1 Pérez Sancho, Marta
A1 Camino Gutiérrez, Eliazar
A1 Gómez Arrones, Vanesa
A1 Carrasco, Juan Jesús
A1 Gabriel Pérez, Jesús de
A1 Serres Dalmau, María Consolacion
A1 Cruz López, Fátima
AB Taylorella asinigenitalis is a non-pathogenic bacteria isolated from the genital tract of donkeys but also a cause of metritis and vaginal discharge in mares. It is closely related to Taylorella equigenitalis, the cause of Contagious Equine Metritis (CEM) in horses, and has been present in different countries in Europe since 1995. Up to date, there are no studies on the prevalence of T. asinigenitalis in the equine or asinine populations in Spain; this is the first report of the presence of T. asinigenitalis in donkeys (Equus asinus) from different breeds in three regions of Spain. A total of 106 healthy animals of three different Spanish donkey breeds: Andaluza (26), Majorera (12) and Zamorano-Leonés (68) were sampled between June and July 2017 and a real-time PCR was used to detect T. asinigenitalis in all samples. A total of 39/221 (17,65 %) samples from 22/106 (20,75 %) animals yielded a positive result and were further characterized by MLST; an allelic profile and Sequence Type (ST) could be assigned to 11 of the 39 positive samples, resulting in four novel STs and no clonal complexes within the PubMLST database. There were statistically significant differences in the percentage of positive animals by breed and sex, and also in the variability of STs between farms. Breeding management would have an influence on the percentage of positives in a farm; artificial insemination and separating jacks from jennies should be implemented. Further studies to detect and characterize T. asinigenitalis in donkeys and horses from Spain would be required to obtain a broader epidemiological picture in this country.
PB Elsevier
SN 03781135
YR 2023
FD 2023-11-24
LK https://hdl.handle.net/20.500.14352/72778
UL https://hdl.handle.net/20.500.14352/72778
LA eng
NO CRUE-CSIC (Acuerdos Transformativos 2022)
NO Universidad Complutense de Madrid Predoctorales
DS Docta Complutense
RD 20 may 2024