TY  - GEN
AU  - Morellon-Sterling, Roberto
AU  - Bolívar Bolívar, Juan Manuel
AU  - Fernandez-Lafuente, Roberto
PY  - 2022
DO  - 10.1016/j.ijbiomac.2022.08.155
SN  - 0141-8130
UR  - https://hdl.handle.net/20.500.14352/114622
AB  - The immobilization of ficin (a cysteinyl proteases) on vinyl sulfone agarose produced its almost full inactivation. It was observed that the incubation of the free and immobilized enzyme in β-mercaptoethanol produced a 20 % of enzyme activity...
LA  - eng
PB  - Elsevier
KW  - Reversible Cys protection
KW  - Cysteinyl enzymes immobilization
KW  - Enzyme stabilization
TI  - Switch off/switch on of a cysteinyl protease as a way to preserve the active catalytic group by modification with a reversible covalent thiol modifier: Immobilization of ficin on vinyl-sulfone activated supports
TY  - working paper
VL  - 220
ER  -