RT Journal Article
T1 A Highly Sensitive Method for the Detection of Hydrolyzed Gluten in Beer Samples Using LFIA
A1 Segura, Verónica
A1 Siglez, Miguel Ángel
A1 Ruiz Carnicer, Ángela
A1 Martín Cabrejas, Izaskun
A1 van der Hofstadt, María
A1 Mellado, Encarnación
A1 Comino, Isabel
A1 Sousa, Carolina
AB Most gluten analysis methods have been developed to detect intact gluten, but they have shown limitations in certain foods and beverages in which gluten proteins are hydrolyzed. Methods based on G12/A1 moAbs detect the sequences of gluten immunogenic peptides (GIP), which are the main contributors to the immune response of celiac disease (CD). Immunogenic sequences with tandem epitopes for G12/A1 have been found in beers with <20 mg/kg gluten, which could be consumed by CD patients according to the Codex Alimentarius. Therefore, an accurate method for the estimation of the immunogenicity of a beer is to use two moAbs that can recognize celiac T cell epitopes comprising most of the immunogenic response. Here, a specific and sensitive method based on G12/A1 LFIA was developed to detect GIP in beers labeled gluten-free or with low gluten content, with an LOD of 0.5 mg/kg. A total of 107 beers were analyzed, of those 6.5% showed levels higher than 20 mg/kg gluten and 29% showed levels above the LOD. In addition, G12/A1 LFIA detected gluten in 15 more beer samples than competitive ELISA with another antibody. Despite their labeling, these beers contained GIP which may cause symptoms and/or intestinal damage in CD patients.
PB MPDI
SN 2304-8158
YR 2022
FD 2022-12-28
LK https://hdl.handle.net/20.500.14352/73197
UL https://hdl.handle.net/20.500.14352/73197
LA eng
NO Technological Corporation of Andalusia (CTA) and CDTI
DS Docta Complutense
RD 20 abr 2025