RT Journal Article
T1 Investigation on the relationship between lipid composition and structure in model membranes composed of extracted natural phospholipids
A1 Santamaria, Andreas
A1 Batchu, Krishna
A1 Fragneto, Giovanna
A1 Laux, Valérie
A1 Haertlein, Michael
A1 Darwish, Tamim
A1 Russell, Robert
A1 Zaccai, Nathan
A1 Guzmán Solís, Eduardo
A1 Maestro, Armando
AB HypothesisUnravelling the structural diversity of cellular membranes is a paramount challenge in life sciences. In particular, lipid composition affects the membrane collective behaviour, and its interactions with other biological molecules.ExperimentsHere, the relationship between membrane composition and resultant structural features was investigated by surface pressure-area isotherms, Brewster angle microscopy and neutron reflectometry on in vitro membrane models of the mammalian plasma and endoplasmic-reticulum-Golgi intermediate compartment membranes in the form of Langmuir monolayers. Natural extracted yeast lipids were used because, unlike synthetic lipids, the acyl chain saturation pattern of yeast and mammalian lipids are similar.FindingsThe structure of the model membranes, orthogonal to the plane of the membrane, as well as their lateral packing, were found to depend strongly on their specific composition, with cholesterol having a major influence on the in-plane morphology, yielding a coexistence of liquid-order and liquid-disorder phases.
PB Elsevier
SN 0021-9797
YR 2023
FD 2023
LK https://hdl.handle.net/20.500.14352/95186
UL https://hdl.handle.net/20.500.14352/95186
LA eng
NO Santamaria, Andreas, et al. «Investigation on the Relationship between Lipid Composition and Structure in Model Membranes Composed of Extracted Natural Phospholipids». Journal of Colloid and Interface Science, vol. 637, mayo de 2023, pp. 55-66. https://doi.org/10.1016/j.jcis.2023.01.043.
NO Eusko Jaurlaritza
NO The National Collaborative Research Infrastructure Strategy (Australia)
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Institut Laue-Langevin (Francia)
NO Wellcome Trust (Reino Unido)
DS Docta Complutense
RD 6 oct 2024