RT Journal Article
T1 Maternal and infant immune repertoire sequencing analysis identifies distinct IG and TCR development in term and preterm infants
A1 Le, Brian L.
A1 Sper, Renan
A1 Nielsen, Sandra Cathrine Abel
A1 Pineda Sanjuan, Silvia
A1 Nguyen, Quoc-Hung
A1 Lee, Ji-Yeun
A1 Boyd, Scott D.
A1 MacKenzie, Tippi C.
A1 Sirota, Marina
A2 Bishop, Gail A.
AB Preterm labor (PTL) is the leading cause of neonatal morbidity and mortality worldwide. Whereas many studies have investigated the maternal immune responses that cause PTL, fetal immune cell activation has recently been raised as an important contributor to the pathogenesis of PTL. In this study, we analyzed lymphocyte receptor repertoires in maternal and cord blood from 14 term and 10 preterm deliveries, hypothesizing that the high prevalence of infection in patients with PTL may result in specific changes in the T cell and B cell repertoires. We analyzed TCR b-chain (TCR-b) and IgH diversity, CDR3 lengths, clonal sharing, and preferential usage of variable and joining gene segments. Both TCR-b and IgH repertoires had shorter CDR3s compared with those in maternal blood. In cord blood samples, we found that CDR3 lengths correlated with gestational age, with shorter CDR3s in preterm neonates suggesting a less developed repertoire. Preterm cord blood displayed preferential usage of a number of genes. In preterm pregnancies, we observed significantly higher prevalence of convergent clones between mother/baby pairs than in term pregnancies. Together, our results suggest the repertoire of preterm infants displays a combination of immature features and convergence with maternal TCR-b clones compared with that of term infants. The higher clonal convergence in PTL could represent mother and fetus both responding to a shared stimulus like an infection. These data provide a detailed analysis of the maternaletal immune repertoire in term and preterm patients and contribute to a better understanding of neonate immune repertoire development and potential changes associated with PTL
PB American Association of Immunologists
SN 0022-1767
YR 2021
FD 2021-11-15
LK https://hdl.handle.net/20.500.14352/102658
UL https://hdl.handle.net/20.500.14352/102658
LA eng
NO Le, B.L. et al. (2021) «Maternal and Infant Immune Repertoire Sequencing Analysis Identifies Distinct Ig and TCR Development in Term and Preterm Infants», Journal of Immunology, 207(10), pp. 2445-2455. doi:10.4049/JIMMUNOL.2100566.
NO Burroughs Wellcome Fund
NO National Institutes of Health
NO Ulla og Mogens Folmer Andersens Fond
DS Docta Complutense
RD 7 abr 2025