RT Journal Article
T1 Rod-Like Microglia Are Restricted to Eyes with Laser-Induced Ocular Hypertension but Absent from the Microglial Changes in the Contralateral Untreated Eye
A1 Hoz Montañana, María Rosa De
A1 Gallego Collado, Beatriz Isabel
A1 Ramírez Sebastián, Ana Isabel
A1 Rojas López, María Blanca
A1 Salazar Corral, Juan José
A1 Valiente Soriano, Francisco Javier
A1 Avilés Trigueros, Marcelino
A1 Villegas Pérez, María Paz
A1 Vidal Sanz, Manuel
A1 Triviño Casado, Alberto
A1 Ramírez Sebastián, José Manuel
AB In the mouse model of unilateral laser-induced ocular hypertension (OHT) the microglia in both the treated and the normotensive untreated contralateral eye have morphological signs of activation and up-regulation of MHC-II expression in comparison with naive. In the brain, rod-like microglia align to less-injured neurons in an effort to limit damage. We investigate whether: i) microglial activation is secondary to laser injury or to a higher IOP and; ii) the presence of rod-like microglia is related to OHT. Three groups of mice were used: age-matched control (naive, n=15); and two lasered: limbal (OHT, n=15); and non-draining portion of the sclera (scleral, n=3). In the lasered animals, treated eyes as well as contralateral eyes were analysed. Retinal whole-mounts were immunostained with antibodies against, Iba-1, NF-200, MHC-II, CD86, CD68 and Ym1. In the scleral group (normal ocular pressure) no microglial signs of activation were found. Similarly to naive eyes, OHT-eyes and their contralateral eyes had ramified microglia in the nerve-fibre layer related to the blood vessel. However, only eyes with OHT had rod-like microglia that aligned end-to-end, coupling to form trains of multiple cells running parallel to axons in the retinal surface. Rod-like microglia were CD68+ and were related to retinal ganglion cells (RGCs) showing signs of degeneration (NF-200+ RGCs). Although MHC-II expression was up-regulated in the microglia of the NFL both in OHT-eyes and their contralateral eyes, no expression of CD86 and Ym1 was detected in ramified or in rod-like microglia. After 15 days of unilateral lasering of the limbal and the non-draining portion of the sclera, activated microglia was restricted to OHT-eyes and their contralateral eyes. However, rod-like microglia were restricted to eyes with OHT and degenerated NF-200+ RGCs and were absent from their contralateral eyes. Thus, rod-like microglia seem be related to the neurodegeneration associated with HTO.
PB Public Library Science
SN 1932-6203
YR 2013
FD 2013-12-18
LK https://hdl.handle.net/20.500.14352/34759
UL https://hdl.handle.net/20.500.14352/34759
LA eng
NO Hoz Montañana, M. R., Gallego Collado, B. I., Ramírez Sebastián, A. I. et al. «Rod-Like Microglia Are Restricted to Eyes with Laser-Induced Ocular Hypertension but Absent from the Microglial Changes in the Contralateral Untreated Eye». PLOS ONE, vol. 8, n.o 12, dic de 2013, p. e83733. PLoS Journals, https://doi.org/10.1371/journal.pone.0083733.
NO © 2013 de Hoz et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permitsunrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
NO Instituto de Salud Carlos III/Ministerio de Ciencia, Innovación y Universidades (España)
NO Ministerio de Economía, Comercio y Empresa (España)
NO Gobierno Regional de Murcia, Fundación Séneca
DS Docta Complutense
RD 9 abr 2025