RT Journal Article
T1 Cerebellar basket cell filtering of Purkinje cell responses elicited by low frequency parallel fibre transmission
A1 Masoli, Stefano
A1 Rizza, Martina Francesca
A1 Soda, Teresa
A1 Sánchez Ponce, Diana
A1 Muñoz Céspedes, Alberto
A1 Prestori, Francesca
A1 D’Angelo, Egidio
AB Basket cells (BC) are inhibitory interneurons of the cerebellar molecular layer (ML) forming peri-somatic synapses on Purkinje cells (PC). BC physiological and computational properties remained poorly understood and not clearly differentiated from those of stellate cells (SC). We identified BCs in acute mouse cerebellar slices and measured their intrinsic excitability and synaptic responsiveness. BCs and SCs were similar in some respects, although BCs showed stronger and faster synaptic excitation in response to parallel fibre (pf) bursts. The analysis of BC inhibition of PCs was extended over a broad parameter space using accurate multi-compartmental computational models. During pf bursts, the BC reduced the PC response at low-frequency, while SCs did it at high-frequency. BC filtering was explained by the engagement of HCN1 channels, which activated slowly during low-frequency BC-PC GABAergic transmission. The increase of input conductance caused by HCN1 channels in the PC soma, by shunting excitatory currents elicited by pfs and travelling toward the axon initial segment (AIS), reduced the PC output frequency. These simulations predict that BC and SC operate in tandem, setting the frequency band of PC transmission through the regulation of PC frequency/response curves.
PB Springer Nature
SN 2045-2322
YR 2025
FD 2025-07-12
LK https://hdl.handle.net/20.500.14352/123480
UL https://hdl.handle.net/20.500.14352/123480
LA eng
NO Masoli, S., Rizza, M. F., Soda, T., Sánchez-Ponce, D., Munoz, A., Prestori, F., & D’Angelo, E. (2025). Cerebellar basket cell filtering of Purkinje cell responses elicited by low frequency parallel fibre transmission. Scientific Reports, 15(1). https://doi.org/10.1038/S41598-025-09964-2
NO T.S. - D.S.P. - A.M. and E.D. - This research has received funding from the European Union’s Horizon 2020 Framework Program for Research and Innovation under the Specific Grant Agreement No. 945539 (Human Brain Project SGA3). M.F.R. - The European Union’s Horizon Europe Programme under the Specific Grant Agreement No. 101147319 (EBRAINS 2.0 Project). S.M. This project/research received funding from the European Union’s Horizon 2020 Framework Programme for Research and Innovation under the Framework Partnership Agreement No. 650003 (HBP FPA).
NO European Commission
DS Docta Complutense
RD 26 feb 2026