RT Journal Article
T1 Oral re-vaccination of Eurasian wild boar with Mycobacterium bovis BCG yields a strong protective response against challenge with a field strain
A1 Gortazar, Christian
A1 Beltrán-Beck, Beatriz
A1 Garrido, Joseba M
A1 Aranaz Martín, Alicia
A1 Sevilla, Iker A
A1 Boadella, Mariana
A1 Lyashchenko, Konstantin P
A1 Galindo, Ruth C
A1 Montoro, Vidal
A1 Juste, Ramón
A1 De la Fuente, Jose
A1 Domínguez Rodríguez, Lucas José
AB BACKGROUNDField vaccination trials with Mycobacterium bovis BCG, an attenuated mutant of M. bovis, are ongoing in Spain, where the Eurasian wild boar (Sus scrofa) is regarded as the main driver of animal tuberculosis (TB). The oral baiting strategy consists in deploying vaccine baits twice each summer, in order to gain access to a high proportion of wild boar piglets. The aim of this study was to assess the response of wild boar to re-vaccination with BCG and to subsequent challenge with an M. bovis field strain.RESULTSBCG re-vaccinated wild boar showed reductions of 75.8% in lesion score and 66.9% in culture score, as compared to unvaccinated controls. Only one of nine vaccinated wild boar had a culture-confirmed lung infection, as compared to seven of eight controls. Serum antibody levels were highly variable and did not differ significantly between BCG re-vaccinated wild boar and controls. Gamma IFN levels differed significantly between BCG re-vaccinated wild boar and controls. The mRNA levels for IL-1b, C3 and MUT were significantly higher in vaccinated wild boar when compared to controls after vaccination and decreased after mycobacterial challenge.CONCLUSIONSOral re-vaccination of wild boar with BCG yields a strong protective response against challenge with a field strain. Moreover, re-vaccination of wild boar with BCG is not counterproductive. These findings are relevant given that re-vaccination is likely to happen under real (field) conditions.
PB BioMedCentral
SN 1746-6148
YR 2014
FD 2014-04-26
LK https://hdl.handle.net/20.500.14352/35278
UL https://hdl.handle.net/20.500.14352/35278
LA eng
NO Unión Europea. FP7
NO Ministerio de Economía y Competitividad (MINECO)
DS Docta Complutense
RD 6 oct 2024