RT Journal Article
T1 Label-free electrochemical immunosensing of glial fibrillary acidic protein (GFAP) at synthesized rGO/MoS2/AgNPs nanocomposite. Application to the determination in human cerebrospinal fluid
A1 García Rodrigo, Lorena
A1 Ramos López, Claudia
A1 Sánchez Tirado, Esther
A1 Agüí Chicharro, María Lourdes
A1 González Cortés, Araceli
A1 Yáñez-Sedeño Orive, Paloma
A1 Pingarrón Carrazón, José Manuel
AB An electrochemical bioplatform involving screen-printed carbon electrodes modified with rGO/MoS2/AgNPs nanocomposites, the covalent immobilization of the specific capture antibody, and label-free detection has been developed for the determination of Glial Fibrillary Acidic Protein (GFAP). The resulting immunosensor profits the benefits of the rGO high conductivity, the pseudo-peroxidase activity of MoS2 and the electrocatalytic effect provided by AgNPs for improving the reduction current responses of hydrogen peroxide at the electrode surface. GFAP is a biomarker of central nervous system injuries has been proposed for the detection and monitoring of neurological diseases as epilepsy, encephalitis, or multiple sclerosis. For the first time, amperometric detection of the immunosensing event was performed by measuring the electrocatalytic response of hydrogen peroxide reduction at the modified electrode. Several techniques including scanning (SEM) and transmission (TEM) electron microscopies were used for the characterization of the synthesized composite whilst electrochemical  impedance spectroscopy (EIS) using the redox probe Fe(CN)63− /4− was employed to evaluate the success of the steps implied in the fabrication of the immunosensor. After optimization of the involved experimental variables, a linear calibration plot for GFAP was constructed over the 0.6–100 ng mL− 1 range, and a detection limit of 0.16 ng mL− 1 was achieved. The developed immunosensor was successfully applied to the determination of GFAP in human cerebrospinal fluid (CSF) of patients diagnosed with encephalitis.
PB Elsevier
SN 0039-9140
YR 2023
FD 2023-12-23
LK https://hdl.handle.net/20.500.14352/113034
UL https://hdl.handle.net/20.500.14352/113034
LA eng
NO Lorena García-Rodrigo, Claudia Ramos-López, Esther Sánchez-Tirado, Lourdes Agüí, Araceli González-Cortés, Paloma Yáñez-Sedeño, José M. Pingarrón, Label-free electrochemical immunosensing of glial fibrillary acidic protein (GFAP) at synthesized rGO/MoS2/AgNPs nanocomposite. Application to the determination in human cerebrospinal fluid, Talanta, Volume 270, 2024, 125597, ISSN 0039-9140, https://doi.org/10.1016/j.talanta.2023.125597. (https://www.sciencedirect.com/science/article/pii/S0039914023013486)
NO Ministerio de Ciencia e Innovación
NO Comunidad de Madrid
DS Docta Complutense
RD 14 dic 2025