RT Journal Article
T1 Candida albicans/Macrophage Biointerface on Human and Porcine Decellularized Adipose Matrices
A1 Cicuéndez Maroto, Mónica
A1 Casarrubios Molina, Laura
A1 Feito Castellano, María José
A1 Madarieta, Iratxe
A1 García Urkia, Nerea
A1 Murua, Olatz
A1 Olalde, Beatriz
A1 Briz, Nerea
A1 Díez Orejas, Rosalía María
A1 Portolés Pérez, María Teresa
AB Macrophages, cells effective in sensing, internalizing and killing Candida albicans, are intertwined with the extracellular matrix (ECM) through different signals, which include the release of specific cytokines. Due to the importance of these interactions, the employment of in vitro models mimicking a fungal infection scenario is essential to evaluate the ECM effects on the macrophage response. In this work, we have analyzed the effects of human and porcine decellularized adipose matrices (DAMs), obtained by either enzymatic or organic solvent treatment, on the macrophage/Candida albicans interface. The present study has allowed us to detect differences on the activation of macrophages cultured on either human- or porcine-derived DAMs, evidencing changes in the macrophage actin cytoskeleton, such as distinct F-actin-rich membrane structures to surround the pathogen. The macrophage morphological changes observed on these four DAMs are key to understand the defense capability of these cells against this fungal pathogen. This work has contributed to the knowledge of the influence that the extracellular matrix and its components can exert on macrophage metabolism, immunocompetence and capacity to respond to the microenvironment in a possible infection scenario.
PB MDPI
SN 2309-608X
YR 2021
FD 2021-05-17
LK https://hdl.handle.net/20.500.14352/7093
UL https://hdl.handle.net/20.500.14352/7093
LA eng
NO Cicuéndez Maroto, M., Casarrubios Molina, L., Feito Castellano, M. J. et al. «Candida Albicans/Macrophage Biointerface on Human and Porcine Decellularized Adipose Matrices». Journal of Fungi, vol. 7, n.o 5, mayo de 2021, p. 392. DOI.org (Crossref), https://doi.org/10.3390/jof7050392.
NO European Union’s Horizon 2020 Research and Innovation Programme (H2020-FETOPEN-2018-2020, NeuroStimSpinal Project, Grant Agreement No. 829060).
DS Docta Complutense
RD 6 abr 2025