RT Journal Article
T1 Reduced expression of galectin-1 and galectin-9 by leucocytes in asthma patients
A1 Sanchez-Cuellar, Silvia
A1 Fuente, Hortensia de la
A1 Cruz Adalia, Aranzazu
A1 Cibrian, Danay
A1 Giron, Rosa
A1 Vara, Alicia
A1 Sanchez-Madrid, Francisco
A1 Lamana Domínguez, Amalia
A1 Ancochea, Julio
AB Accumulating evidence shows that galectins play roles in the initiation and resolution phases of inflammatory responses by promoting anti- or proinflammatory effects. This study investigated the presence of three members of the galectin family (galectin-1, -3 and -9) in induced sputum samples of asthma patients, as well as their possible implication in the immunopathogenesis of human asthma. Levels of interleukin (IL)-5, IL-13, and galectins were determined in leucocytes isolated from induced sputum samples by reverse transcription–polymerase chain reaction (RT–PCR) immunofluorescence and flow cytometry. High levels of IL-5 and IL-13 mRNA were detected in sputum cells from asthma patients. In parallel, immunoregulatory proteins galectin-1 and galectin-9 showed a reduced expression on macrophages from sputum samples compared with cells from healthy donors. In-vitro immunoassays showed that galectin-1 and galectin-9, but not galectin-3, are able to induce the production of IL-10 by peripheral blood mononuclear cells from healthy donors. These findings indicate that macrophages from sputum samples of asthma patients express low levels of galectin-1 and galectin-9, favouring the exacerbated immune response observed in this disease.
PB Oxford Academic
SN 0009-9104
YR 2012
FD 2012
LK https://hdl.handle.net/20.500.14352/94271
UL https://hdl.handle.net/20.500.14352/94271
LA eng
NO S Sanchez-Cuellar, H de la Fuente, A Cruz-Adalia, A Lamana, D Cibrian, R M Giron, A Vara, F Sanchez-Madrid, J Ancochea, Reduced expression of galectin-1 and galectin-9 by leucocytes in asthma patients, Clinical and Experimental Immunology, Volume 170, Issue 3, December 2012, Pages 365–374, https://doi.org/10.1111/j.1365-2249.2012.04665.x
NO Supported in part by EU–Mexico FONCICYT-C002-2009-1 ALA/127249, SAF-2008–02635 and SAF-2011–25834 from the Spanish Ministry of Science and Innovation, INDISNET (Redes Moleculares y Celulares en Enfermedades Inflamatorias) S2011/BMD-2332, MEICA (Molecular and Cellular Mechanisms in Chronic Inflammatory and Autoimmune Diseases, Genoma España) and SEPAR (Sociedad Española de Patología Respiratoria).
NO Consejo Nacional de Humanidades, Ciencias y Tecnologías (México)
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Redes Moleculares y Celulares en Enfermedades Inflamatorias (España)
NO Sociedad Española de Patología Respiratoria
DS Docta Complutense
RD 17 abr 2025