RT Journal Article
T1 Disposable electrochemical biosensors for Brettanomyces bruxellensis and total yeast content in wine based on core-shell magnetic nanoparticles
A1 Villalonga, María
A1 Borisova, Boryana
A1 Arenas, Christian
A1 Villalonga, Anabel
A1 Arévalo-Villena, María
A1 Sánchez Sánchez, Alfredo
A1 Pingarrón Carrazón, José Manuel
A1 Briones-Pérez, Ana
A1 Villalonga Santana, Reynaldo
AB Two different disposable amperometric biosensors for the rapid detection and quantification of Brettanomyces bruxellensis (Brett) and total yeast content in wine were constructed. The biosensing approach implied the use of magnetic capturing elements based on core-shell Fe3O4@SiO2 superparamagnetic nanoparticles (NanoCaptors) functionalized with specific bioreceptors: Concanavalin A (Con A) for total yeast analysis, and anti-Brett polyclonal antibodies for Brett. A Con A-peroxidase conjugate and carbon screen printed electrodes were used as signaling element and sensing interfaces, respectively. The biosensor based on antibody-modified magnetic nanoparticles allowed the amperometric detection of B. bruxellensis in buffer solution and red wine samples in the range of 10 - 106 CFU/mL, with low detection limits of 6 CFU/mL and 8 CFU/mL, respectively. In addition, the use of Con A-linked magnetic nanoparticles as capturing elements allowed determination of total yeast content in buffer solution and red wine in the range of 10 - 106 CFU/mL, with detection limit of 5 CFU/mL. These electrochemical biosensors also exhibited high reproducibility, selectivity and storage stability.
PB Elsevier 
SN 0925-4005
YR 2018
FD 2018
LK https://hdl.handle.net/20.500.14352/92685
UL https://hdl.handle.net/20.500.14352/92685
LA eng
NO Villalonga, M. L., Borisova, B., Arenas, C. B., Villalonga, A., Arévalo-Villena, M., Sánchez, A., Pingarrón, J. M., Briones-Pérez. A., Villalonga, R.* (2019). Disposable electrochemical biosensors for Brettanomyces bruxellensis and total yeast content in wine based on core-shell magnetic nanoparticles. Sensors and Actuators B: Chemical, 279, 15-21.
NO Ministerio de Economia y ́Competitividad (España)
NO Junta de Comunidades de Castilla La Mancha
NO Comunidad de Madrid
DS Docta Complutense
RD 4 jul 2025