RT Journal Article
T1 The self-absorption phenomenon in quantitative Raman spectroscopy and how to correct its effects
A1 Uriarte, Lucas Martinez
A1 Bonales, Laura
A1 Dubessy, Jean
A1 Lobato Fernández, Álvaro
A1 García Baonza, Valentín
A1 Cáceres Alonso, María Mercedes
AB When the wavelength of the Raman scattered light coincides with the absorption spectrum of the sample, a phenomenon known as Raman self-absorption occurs. If the absorption wavelength range matches completely with the Raman wavenumber range for a given excitation line (high absorption), this process causes spectra with poor signal-to-noise-ratio, making the acquisition nearly impossible. When the absorption of the sample and the Raman scattered are partially coincident (low absorption), smaller modifications in the band-profile of the Raman spectrum are expected difficult to be detected. If a different excitation wavelength is available in order to avoid or minimize this phenomenon, a correction method is customary. The correction methods developed so far in quantitative analysis are still very complex and require the knowledge of several sampling parameters. In this work, we present a very simple but reliable method based on the Beer-Lambert law to correct low self-absorption effects. This method uses simple concepts, a straightforward methodology and the concentration of the Raman active component in the liquid matrix is the only parameter required to perform the correction. This method may be extremely useful in different fields in which Raman spectroscopy is used to obtain molecular and structural information from a band profile analysis. As an example, the method is applied here to successfully correct the Raman spectra of different CuSO4 aqueous solutions excited at 532nm.
PB Elsevier
SN 0026-265X
YR 2018
FD 2018
LK https://hdl.handle.net/20.500.14352/92333
UL https://hdl.handle.net/20.500.14352/92333
LA eng
NO Uriarte, L. M.; Bonales, L. J.; Dubessy, J.; Lobato, A.; Baonza, V. G.; Cáceres, M. The self-absorption phenomenon in quantitative Raman spectroscopy and how to correct its effects. Microchemical Journal 2018, 139, 134-138 DOI:10.1016/j.microc.2018.02.013.
NO Ministerio de Economía y Competitividad (España)
NO Ministerio de Ciencia e Innovación (España)
DS Docta Complutense
RD 26 abr 2025