%0 Journal Article
%A Jarillo, Javier
%A Ibarra, Borja
%A Cao García, Francisco Javier
%T DNA replication: In vitro single-molecule manipulation data analysis and models
%D 2021
%U https://hdl.handle.net/20.500.14352/104481
%X DNA replication is a key biochemical process of the cell cycle. In the last years, analysis of in vitro singlemolecule DNA replication events has provided new information that cannot be obtained with ensemblesstudies. Here, we introduce crucial techniques for the proper analysis and modelling of DNA replication in vitro single-molecule manipulation data. Specifically, we review some of the main methods to analyzeand model the real-time kinetics of the two main molecular motors of the replisome: DNA polymerase and DNA helicase. Our goal is to facilitate access to and understanding of these techniques to promotetheiruse in the study of DNA replication at the single-molecule level. A proper analysis of single-molecule data is crucial to obtain a detailed picture of, among others, the kinetics rates, equilibrium contants andconformational changes of the system under study. The techniques presented here have been used or can be adapted to study the operation of other proteins involved in nucleic acids metabolism.
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