RT Journal Article
T1 Macrophage-dependent neutrophil recruitment is impaired under conditions of increased intestinal permeability in JAM-A-deficient mice
A1 Luissint, Anny-Claude
A1 Williams, Holly C.
A1 Kim, Wooki
A1 Flemming, Sven
A1 Hilgarth, Roland S.
A1 O'Leary, Monique N.
A1 Denning, Tomothy L.
A1 Nusrat, Asma
A1 Parkos, Charles A.
A1 Azcutia Criado, Verónica
AB Junctional adhesion molecule-A (JAM-A) is a transmembrane glycoprotein expressed on leukocytes, endothelia, and epithelia that regulates biological processes including barrier function and immune responses. While JAM-A has been reported to facilitate tissue infiltration of leukocytes under inflammatory conditions, the contributions of leukocyte-expressed JAM-A in vivo remain unresolved. We investigated the role of leukocyte-expressed JAM-A in acute peritonitis induced by zymosan, lipopolysaccharide (LPS), or TNFα using mice with selective loss of JAM-A in myelomonocytic cells (LysM-Cre;Jam-afl/fl). Surprisingly, in LysM-Cre;Jam-afl/fl mice, loss of JAM-A did not affect neutrophil (PMN) recruitment into the peritoneum in response to zymosan, LPS, or TNFα although it was significantly reduced in Jam-aKO mice. In parallel, Jam-aKO peritoneal macrophages exhibited diminished CXCL1 chemokine production and decreased activation of NF-kB, whereas those from LysM-Cre;Jam-afl/fl mice were unaffected. Using Villin-Cre;
PB Springer Nature
YR 2019
FD 2019
LK https://hdl.handle.net/20.500.14352/117343
UL https://hdl.handle.net/20.500.14352/117343
LA eng
NO Luissint, AC., Williams, H.C., Kim, W. et al. Macrophage-dependent neutrophil recruitment is impaired under conditions of increased intestinal permeability in JAM-A-deficient mice. Mucosal Immunol 12, 668–678 (2019). https://doi.org/10.1038/s41385-019-0143-7
NO National Institutes of Health (US)
DS Docta Complutense
RD 18 mar 2026